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Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency

Cpf1, a type-V CRISPR-Cas effector endonuclease, exhibits gene-editing activity in human cells through a single RNA-guided approach. Here, we report the design and assessment of an array of 42 types of engineered Acidaminococcus sp. Cpf1 (AsCpf1) CRISPR RNAs (crRNAs) and 5 types of AsCpf1 mRNAs, and...

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Detalles Bibliográficos
Autores principales:	Li, Bin, Zhao, Weiyu, Luo, Xiao, Zhang, Xinfu, Li, Chenglong, Zeng, Chunxi, Dong, Yizhou
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562407/ https://www.ncbi.nlm.nih.gov/pubmed/28840077 http://dx.doi.org/10.1038/s41551-017-0066

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