Cargando…
Three recombinantly expressed apple tyrosinases suggest the amino acids responsible for mono- versus diphenolase activity in plant polyphenol oxidases
Tyrosinases and catechol oxidases belong to the polyphenol oxidase (PPO) enzyme family, which is mainly responsible for the browning of fruits. Three cDNAs encoding PPO pro-enzymes have been cloned from leaves of Malus domestica (apple, MdPPO). The three pro-enzymes MdPPO1-3 were heterologously expr...
Autores principales: | Kampatsikas, Ioannis, Bijelic, Aleksandar, Pretzler, Matthias, Rompel, Annette |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562730/ https://www.ncbi.nlm.nih.gov/pubmed/28821733 http://dx.doi.org/10.1038/s41598-017-08097-5 |
Ejemplares similares
-
In crystallo activity tests with latent apple tyrosinase and two mutants reveal the importance of the mutated sites for polyphenol oxidase activity
por: Kampatsikas, Ioannis, et al.
Publicado: (2017) -
A Peptide‐Induced Self‐Cleavage Reaction Initiates the Activation of Tyrosinase
por: Kampatsikas, Ioannis, et al.
Publicado: (2019) -
Conversion of walnut tyrosinase into a catechol oxidase by site directed mutagenesis
por: Panis, Felix, et al.
Publicado: (2020) -
Identification of Amino Acid Residues Responsible for C−H Activation in Type‐III Copper Enzymes by Generating Tyrosinase Activity in a Catechol Oxidase
por: Kampatsikas, Ioannis, et al.
Publicado: (2020) -
Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity
por: Kampatsikas, Ioannis, et al.
Publicado: (2019)