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Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells

Earlier investigations have revealed that tumor cells undergo metabolic reprogramming and mainly derive their cellular energy from aerobic glycolysis rather than oxidative phosphorylation even in the presence of oxygen. However, recent studies have shown that certain cancer cells display increased o...

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Autores principales: Dar, Sajad, Chhina, Jasdeep, Mert, Ismail, Chitale, Dhananjay, Buekers, Thomas, Kaur, Hareena, Giri, Shailendra, Munkarah, Adnan, Rattan, Ramandeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562731/
https://www.ncbi.nlm.nih.gov/pubmed/28821788
http://dx.doi.org/10.1038/s41598-017-09206-0
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author Dar, Sajad
Chhina, Jasdeep
Mert, Ismail
Chitale, Dhananjay
Buekers, Thomas
Kaur, Hareena
Giri, Shailendra
Munkarah, Adnan
Rattan, Ramandeep
author_facet Dar, Sajad
Chhina, Jasdeep
Mert, Ismail
Chitale, Dhananjay
Buekers, Thomas
Kaur, Hareena
Giri, Shailendra
Munkarah, Adnan
Rattan, Ramandeep
author_sort Dar, Sajad
collection PubMed
description Earlier investigations have revealed that tumor cells undergo metabolic reprogramming and mainly derive their cellular energy from aerobic glycolysis rather than oxidative phosphorylation even in the presence of oxygen. However, recent studies have shown that certain cancer cells display increased oxidative phosphorylation or high metabolically active phenotype. Cellular bioenergetic profiling of 13 established and 12 patient derived ovarian cancer cell lines revealed significant bioenergetics diversity. The bioenergetics phenotype of ovarian cancer cell lines correlated with functional phenotypes of doubling time and oxidative stress. Interestingly, chemosensitive cancer cell lines (A2780 and PEO1) displayed a glycolytic phenotype while their chemoresistant counterparts (C200 and PEO4) exhibited a high metabolically active phenotype with the ability to switch between oxidative phosphorylation or glycolysis. The chemosensitive cancer cells could not survive glucose deprivation, while the chemoresistant cells displayed adaptability. In the patient derived ovarian cancer cells, a similar correlation was observed between a high metabolically active phenotype and chemoresistance. Thus, ovarian cancer cells seem to display heterogeneity in using glycolysis or oxidative phosphorylation as an energy source. The flexibility in using different energy pathways may indicate a survival adaptation to achieve a higher ‘cellular fitness’ that may be also associated with chemoresistance.
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spelling pubmed-55627312017-08-21 Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells Dar, Sajad Chhina, Jasdeep Mert, Ismail Chitale, Dhananjay Buekers, Thomas Kaur, Hareena Giri, Shailendra Munkarah, Adnan Rattan, Ramandeep Sci Rep Article Earlier investigations have revealed that tumor cells undergo metabolic reprogramming and mainly derive their cellular energy from aerobic glycolysis rather than oxidative phosphorylation even in the presence of oxygen. However, recent studies have shown that certain cancer cells display increased oxidative phosphorylation or high metabolically active phenotype. Cellular bioenergetic profiling of 13 established and 12 patient derived ovarian cancer cell lines revealed significant bioenergetics diversity. The bioenergetics phenotype of ovarian cancer cell lines correlated with functional phenotypes of doubling time and oxidative stress. Interestingly, chemosensitive cancer cell lines (A2780 and PEO1) displayed a glycolytic phenotype while their chemoresistant counterparts (C200 and PEO4) exhibited a high metabolically active phenotype with the ability to switch between oxidative phosphorylation or glycolysis. The chemosensitive cancer cells could not survive glucose deprivation, while the chemoresistant cells displayed adaptability. In the patient derived ovarian cancer cells, a similar correlation was observed between a high metabolically active phenotype and chemoresistance. Thus, ovarian cancer cells seem to display heterogeneity in using glycolysis or oxidative phosphorylation as an energy source. The flexibility in using different energy pathways may indicate a survival adaptation to achieve a higher ‘cellular fitness’ that may be also associated with chemoresistance. Nature Publishing Group UK 2017-08-18 /pmc/articles/PMC5562731/ /pubmed/28821788 http://dx.doi.org/10.1038/s41598-017-09206-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dar, Sajad
Chhina, Jasdeep
Mert, Ismail
Chitale, Dhananjay
Buekers, Thomas
Kaur, Hareena
Giri, Shailendra
Munkarah, Adnan
Rattan, Ramandeep
Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title_full Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title_fullStr Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title_full_unstemmed Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title_short Bioenergetic Adaptations in Chemoresistant Ovarian Cancer Cells
title_sort bioenergetic adaptations in chemoresistant ovarian cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5562731/
https://www.ncbi.nlm.nih.gov/pubmed/28821788
http://dx.doi.org/10.1038/s41598-017-09206-0
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