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Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563041/ https://www.ncbi.nlm.nih.gov/pubmed/28821297 http://dx.doi.org/10.1186/s12944-017-0550-2 |
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author | Luna-Luna, María Cruz-Robles, David Ávila-Vanzzini, Nydia Herrera-Alarcón, Valentín Martínez-Reding, Jesús Criales-Vera, Sergio Sandoval-Zárate, Julio Vargas-Barrón, Jesús Martínez-Sánchez, Carlos Tovar-Palacio, Armando Roberto Fragoso, José Manuel Carreón-Torres, Elizabeth Vargas-Alarcón, Gilberto Pérez-Méndez, Óscar |
author_facet | Luna-Luna, María Cruz-Robles, David Ávila-Vanzzini, Nydia Herrera-Alarcón, Valentín Martínez-Reding, Jesús Criales-Vera, Sergio Sandoval-Zárate, Julio Vargas-Barrón, Jesús Martínez-Sánchez, Carlos Tovar-Palacio, Armando Roberto Fragoso, José Manuel Carreón-Torres, Elizabeth Vargas-Alarcón, Gilberto Pérez-Méndez, Óscar |
author_sort | Luna-Luna, María |
collection | PubMed |
description | BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. METHODS: To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. RESULTS: EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque CONCLUSION: OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0550-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5563041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55630412017-08-21 Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses Luna-Luna, María Cruz-Robles, David Ávila-Vanzzini, Nydia Herrera-Alarcón, Valentín Martínez-Reding, Jesús Criales-Vera, Sergio Sandoval-Zárate, Julio Vargas-Barrón, Jesús Martínez-Sánchez, Carlos Tovar-Palacio, Armando Roberto Fragoso, José Manuel Carreón-Torres, Elizabeth Vargas-Alarcón, Gilberto Pérez-Méndez, Óscar Lipids Health Dis Research BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. METHODS: To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. RESULTS: EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque CONCLUSION: OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0550-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-18 /pmc/articles/PMC5563041/ /pubmed/28821297 http://dx.doi.org/10.1186/s12944-017-0550-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Luna-Luna, María Cruz-Robles, David Ávila-Vanzzini, Nydia Herrera-Alarcón, Valentín Martínez-Reding, Jesús Criales-Vera, Sergio Sandoval-Zárate, Julio Vargas-Barrón, Jesús Martínez-Sánchez, Carlos Tovar-Palacio, Armando Roberto Fragoso, José Manuel Carreón-Torres, Elizabeth Vargas-Alarcón, Gilberto Pérez-Méndez, Óscar Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title_full | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title_fullStr | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title_full_unstemmed | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title_short | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
title_sort | differential expression of osteopontin, and osteoprotegerin mrna in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with hdl subclasses |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563041/ https://www.ncbi.nlm.nih.gov/pubmed/28821297 http://dx.doi.org/10.1186/s12944-017-0550-2 |
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