Cargando…

Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses

BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated...

Descripción completa

Detalles Bibliográficos
Autores principales: Luna-Luna, María, Cruz-Robles, David, Ávila-Vanzzini, Nydia, Herrera-Alarcón, Valentín, Martínez-Reding, Jesús, Criales-Vera, Sergio, Sandoval-Zárate, Julio, Vargas-Barrón, Jesús, Martínez-Sánchez, Carlos, Tovar-Palacio, Armando Roberto, Fragoso, José Manuel, Carreón-Torres, Elizabeth, Vargas-Alarcón, Gilberto, Pérez-Méndez, Óscar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563041/
https://www.ncbi.nlm.nih.gov/pubmed/28821297
http://dx.doi.org/10.1186/s12944-017-0550-2
_version_ 1783258062936604672
author Luna-Luna, María
Cruz-Robles, David
Ávila-Vanzzini, Nydia
Herrera-Alarcón, Valentín
Martínez-Reding, Jesús
Criales-Vera, Sergio
Sandoval-Zárate, Julio
Vargas-Barrón, Jesús
Martínez-Sánchez, Carlos
Tovar-Palacio, Armando Roberto
Fragoso, José Manuel
Carreón-Torres, Elizabeth
Vargas-Alarcón, Gilberto
Pérez-Méndez, Óscar
author_facet Luna-Luna, María
Cruz-Robles, David
Ávila-Vanzzini, Nydia
Herrera-Alarcón, Valentín
Martínez-Reding, Jesús
Criales-Vera, Sergio
Sandoval-Zárate, Julio
Vargas-Barrón, Jesús
Martínez-Sánchez, Carlos
Tovar-Palacio, Armando Roberto
Fragoso, José Manuel
Carreón-Torres, Elizabeth
Vargas-Alarcón, Gilberto
Pérez-Méndez, Óscar
author_sort Luna-Luna, María
collection PubMed
description BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. METHODS: To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. RESULTS: EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque CONCLUSION: OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0550-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5563041
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-55630412017-08-21 Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses Luna-Luna, María Cruz-Robles, David Ávila-Vanzzini, Nydia Herrera-Alarcón, Valentín Martínez-Reding, Jesús Criales-Vera, Sergio Sandoval-Zárate, Julio Vargas-Barrón, Jesús Martínez-Sánchez, Carlos Tovar-Palacio, Armando Roberto Fragoso, José Manuel Carreón-Torres, Elizabeth Vargas-Alarcón, Gilberto Pérez-Méndez, Óscar Lipids Health Dis Research BACKGROUND: Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. METHODS: To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. RESULTS: EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque CONCLUSION: OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0550-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-18 /pmc/articles/PMC5563041/ /pubmed/28821297 http://dx.doi.org/10.1186/s12944-017-0550-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Luna-Luna, María
Cruz-Robles, David
Ávila-Vanzzini, Nydia
Herrera-Alarcón, Valentín
Martínez-Reding, Jesús
Criales-Vera, Sergio
Sandoval-Zárate, Julio
Vargas-Barrón, Jesús
Martínez-Sánchez, Carlos
Tovar-Palacio, Armando Roberto
Fragoso, José Manuel
Carreón-Torres, Elizabeth
Vargas-Alarcón, Gilberto
Pérez-Méndez, Óscar
Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title_full Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title_fullStr Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title_full_unstemmed Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title_short Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
title_sort differential expression of osteopontin, and osteoprotegerin mrna in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with hdl subclasses
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563041/
https://www.ncbi.nlm.nih.gov/pubmed/28821297
http://dx.doi.org/10.1186/s12944-017-0550-2
work_keys_str_mv AT lunalunamaria differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT cruzroblesdavid differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT avilavanzzininydia differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT herreraalarconvalentin differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT martinezredingjesus differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT crialesverasergio differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT sandovalzaratejulio differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT vargasbarronjesus differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT martinezsanchezcarlos differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT tovarpalacioarmandoroberto differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT fragosojosemanuel differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT carreontorreselizabeth differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT vargasalarcongilberto differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses
AT perezmendezoscar differentialexpressionofosteopontinandosteoprotegerinmrnainepicardialadiposetissuebetweenpatientswithseverecoronaryarterydiseaseandaorticvalvularstenosisassociationwithhdlsubclasses