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Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply
Nitrogen (N) is critical for zinc (Zn) absorption into plant roots; this in turn allows for Zn accumulation and biofortification of grain in winter wheat (Triticum aestivum L.), an important food crop. However, little is known about root morphology and subcellular Zn distribution in response to N tr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563362/ https://www.ncbi.nlm.nih.gov/pubmed/28868060 http://dx.doi.org/10.3389/fpls.2017.01435 |
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author | Nie, Zhaojun Zhao, Peng Wang, Jia Li, Jinfeng Liu, Hongen |
author_facet | Nie, Zhaojun Zhao, Peng Wang, Jia Li, Jinfeng Liu, Hongen |
author_sort | Nie, Zhaojun |
collection | PubMed |
description | Nitrogen (N) is critical for zinc (Zn) absorption into plant roots; this in turn allows for Zn accumulation and biofortification of grain in winter wheat (Triticum aestivum L.), an important food crop. However, little is known about root morphology and subcellular Zn distribution in response to N treatment at different levels of Zn supply. In this study, two nutrient solution culture experiments were conducted to examine Zn accumulation, Zn absorption kinetics, root morphology, and Zn subcellular distribution in wheat seedlings pre-cultured with different N concentrations. The results showed positive correlations between N and Zn concentrations, and N and Zn accumulation, respectively. The findings suggested that an increase in N supply enhanced root absorption and the root-to-shoot transport of Zn. Nitrogen combined with the high Zn (Zn(10)) treatment increased the Zn concentration and consequently its accumulation in both shoots and roots. The maximum influx rate (V(max)), root length, surface area, and volume of 14-d-old seedlings, and root growth from 7 to 14 d in the medium N (N(7.5)) treatment were higher, but the Michaelis constant (K(m)) and minimum equilibrium concentrations (C(min)) in this treatment were lower than those in the low (N(0.05)) and high (N(15)) N treatments, when Zn was supplied at a high level (Zn(10)). Meanwhile, there were no pronounced differences in the above root traits between the N(0.05)Zn(0) and N(7.5)Zn(10) treatments. An increase in N supply decreased Zn in cell walls and cell organelles, while it increased Zn in the root soluble fraction. In leaves, an increase in N supply significantly decreased Zn in cell walls and the soluble fraction, while it increased Zn in cell organelles under Zn deficiency, but increased Zn distribution in the soluble fraction under medium and high Zn treatments. Therefore, a combination of medium N and high Zn treatments enhanced Zn absorption, apparently by enhancing Zn membrane transport and stimulating root development in winter wheat. An increase in N supply was beneficial in terms of achieving a balanced distribution of Zn subcellular fractions, thus enhancing Zn translocation to shoots, while maintaining normal metabolism. |
format | Online Article Text |
id | pubmed-5563362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55633622017-09-01 Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply Nie, Zhaojun Zhao, Peng Wang, Jia Li, Jinfeng Liu, Hongen Front Plant Sci Plant Science Nitrogen (N) is critical for zinc (Zn) absorption into plant roots; this in turn allows for Zn accumulation and biofortification of grain in winter wheat (Triticum aestivum L.), an important food crop. However, little is known about root morphology and subcellular Zn distribution in response to N treatment at different levels of Zn supply. In this study, two nutrient solution culture experiments were conducted to examine Zn accumulation, Zn absorption kinetics, root morphology, and Zn subcellular distribution in wheat seedlings pre-cultured with different N concentrations. The results showed positive correlations between N and Zn concentrations, and N and Zn accumulation, respectively. The findings suggested that an increase in N supply enhanced root absorption and the root-to-shoot transport of Zn. Nitrogen combined with the high Zn (Zn(10)) treatment increased the Zn concentration and consequently its accumulation in both shoots and roots. The maximum influx rate (V(max)), root length, surface area, and volume of 14-d-old seedlings, and root growth from 7 to 14 d in the medium N (N(7.5)) treatment were higher, but the Michaelis constant (K(m)) and minimum equilibrium concentrations (C(min)) in this treatment were lower than those in the low (N(0.05)) and high (N(15)) N treatments, when Zn was supplied at a high level (Zn(10)). Meanwhile, there were no pronounced differences in the above root traits between the N(0.05)Zn(0) and N(7.5)Zn(10) treatments. An increase in N supply decreased Zn in cell walls and cell organelles, while it increased Zn in the root soluble fraction. In leaves, an increase in N supply significantly decreased Zn in cell walls and the soluble fraction, while it increased Zn in cell organelles under Zn deficiency, but increased Zn distribution in the soluble fraction under medium and high Zn treatments. Therefore, a combination of medium N and high Zn treatments enhanced Zn absorption, apparently by enhancing Zn membrane transport and stimulating root development in winter wheat. An increase in N supply was beneficial in terms of achieving a balanced distribution of Zn subcellular fractions, thus enhancing Zn translocation to shoots, while maintaining normal metabolism. Frontiers Media S.A. 2017-08-18 /pmc/articles/PMC5563362/ /pubmed/28868060 http://dx.doi.org/10.3389/fpls.2017.01435 Text en Copyright © 2017 Nie, Zhao, Wang, Li and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Nie, Zhaojun Zhao, Peng Wang, Jia Li, Jinfeng Liu, Hongen Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title | Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title_full | Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title_fullStr | Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title_full_unstemmed | Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title_short | Absorption Kinetics and Subcellular Fractionation of Zinc in Winter Wheat in Response to Nitrogen Supply |
title_sort | absorption kinetics and subcellular fractionation of zinc in winter wheat in response to nitrogen supply |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563362/ https://www.ncbi.nlm.nih.gov/pubmed/28868060 http://dx.doi.org/10.3389/fpls.2017.01435 |
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