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CLEC5A is a critical receptor in innate immunity against Listeria infection
The C-type lectin member 5A (CLEC5A) is a pattern recognition receptor for members of the Flavivirus family and has critical functions in response to dengue virus and Japanese encephalitis virus. Here we show that CLEC5A is involved in neutrophil extracellular trap formation and the production of re...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563510/ https://www.ncbi.nlm.nih.gov/pubmed/28824166 http://dx.doi.org/10.1038/s41467-017-00356-3 |
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author | Chen, Szu-Ting Li, Fei-Ju Hsu, Tzy-yun Liang, Shu-Mei Yeh, Yi-Chen Liao, Wen-Yu Chou, Teh-Ying Chen, Nien-Jun Hsiao, Michael Yang, Wen-Bin Hsieh, Shie-Liang |
author_facet | Chen, Szu-Ting Li, Fei-Ju Hsu, Tzy-yun Liang, Shu-Mei Yeh, Yi-Chen Liao, Wen-Yu Chou, Teh-Ying Chen, Nien-Jun Hsiao, Michael Yang, Wen-Bin Hsieh, Shie-Liang |
author_sort | Chen, Szu-Ting |
collection | PubMed |
description | The C-type lectin member 5A (CLEC5A) is a pattern recognition receptor for members of the Flavivirus family and has critical functions in response to dengue virus and Japanese encephalitis virus. Here we show that CLEC5A is involved in neutrophil extracellular trap formation and the production of reactive oxygen species and proinflammatory cytokines in response to Listeria monocytogenes. Inoculation of Clec5a (−/−) mice with L. monocytogenes causes rapid bacterial spreading, increased bacterial loads in the blood and liver, and severe liver necrosis. In these mice, IL-1β, IL-17A, and TNF expression is inhibited, CCL2 is induced, and large numbers of CD11b(+)Ly6C(hi)CCR2(hi)CX3CR1(low) inflammatory monocytes infiltrate the liver. By day 5 of infection, these mice also have fewer IL-17A(+) γδ T cells, severe liver necrosis and a higher chance of fatality. Thus, CLEC5A has a pivotal function in the activation of multiple aspects of innate immunity against bacterial invasion. |
format | Online Article Text |
id | pubmed-5563510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55635102017-08-28 CLEC5A is a critical receptor in innate immunity against Listeria infection Chen, Szu-Ting Li, Fei-Ju Hsu, Tzy-yun Liang, Shu-Mei Yeh, Yi-Chen Liao, Wen-Yu Chou, Teh-Ying Chen, Nien-Jun Hsiao, Michael Yang, Wen-Bin Hsieh, Shie-Liang Nat Commun Article The C-type lectin member 5A (CLEC5A) is a pattern recognition receptor for members of the Flavivirus family and has critical functions in response to dengue virus and Japanese encephalitis virus. Here we show that CLEC5A is involved in neutrophil extracellular trap formation and the production of reactive oxygen species and proinflammatory cytokines in response to Listeria monocytogenes. Inoculation of Clec5a (−/−) mice with L. monocytogenes causes rapid bacterial spreading, increased bacterial loads in the blood and liver, and severe liver necrosis. In these mice, IL-1β, IL-17A, and TNF expression is inhibited, CCL2 is induced, and large numbers of CD11b(+)Ly6C(hi)CCR2(hi)CX3CR1(low) inflammatory monocytes infiltrate the liver. By day 5 of infection, these mice also have fewer IL-17A(+) γδ T cells, severe liver necrosis and a higher chance of fatality. Thus, CLEC5A has a pivotal function in the activation of multiple aspects of innate immunity against bacterial invasion. Nature Publishing Group UK 2017-08-21 /pmc/articles/PMC5563510/ /pubmed/28824166 http://dx.doi.org/10.1038/s41467-017-00356-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chen, Szu-Ting Li, Fei-Ju Hsu, Tzy-yun Liang, Shu-Mei Yeh, Yi-Chen Liao, Wen-Yu Chou, Teh-Ying Chen, Nien-Jun Hsiao, Michael Yang, Wen-Bin Hsieh, Shie-Liang CLEC5A is a critical receptor in innate immunity against Listeria infection |
title | CLEC5A is a critical receptor in innate immunity against Listeria infection |
title_full | CLEC5A is a critical receptor in innate immunity against Listeria infection |
title_fullStr | CLEC5A is a critical receptor in innate immunity against Listeria infection |
title_full_unstemmed | CLEC5A is a critical receptor in innate immunity against Listeria infection |
title_short | CLEC5A is a critical receptor in innate immunity against Listeria infection |
title_sort | clec5a is a critical receptor in innate immunity against listeria infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563510/ https://www.ncbi.nlm.nih.gov/pubmed/28824166 http://dx.doi.org/10.1038/s41467-017-00356-3 |
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