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MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells
BACKGROUND: MicroRNA-7 (miR-7) has been observed as a potent tumour suppressor in multiple cancer types including breast cancer. The aim of this study was to investigate the response sensitivities of metastatic breast cancer cells to miR-7 and the roles of miR-7 in the interaction of endothelial cel...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563947/ https://www.ncbi.nlm.nih.gov/pubmed/28571043 http://dx.doi.org/10.1038/bjc.2017.156 |
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author | Cui, Yu-Xin Bradbury, Robyn Flamini, Valentina Wu, Bo Jordan, Nicola Jiang, Wen G |
author_facet | Cui, Yu-Xin Bradbury, Robyn Flamini, Valentina Wu, Bo Jordan, Nicola Jiang, Wen G |
author_sort | Cui, Yu-Xin |
collection | PubMed |
description | BACKGROUND: MicroRNA-7 (miR-7) has been observed as a potent tumour suppressor in multiple cancer types including breast cancer. The aim of this study was to investigate the response sensitivities of metastatic breast cancer cells to miR-7 and the roles of miR-7 in the interaction of endothelial cells and metastatic cancer cells. METHODS: Expression profile of miRNAs in a breast cancer specimen cohort and breast cancer cells were determined using real-time quantitative miRNA assays. Effect of the altering expression of miR-7 on migration, invasion, proliferation, interaction and underlying molecular mechanism of breast cancer cells and endothelial cells was investigated after treatment with the synthesised mimic of miR-7. Luciferase activity analysis was performed to validate Wave-3 as a novel target of miR-7. RESULTS: miR-7 expression was negatively correlated with the stage, grade and survival of the breast cancer patients. There was also differential expression of miRNAs including miR-7 in the breast cancer cells. The synthesised mimic of miR-7 inhibits the motility and wound healing potential of breast cancer cells. The highly metastatic MDA-MB-231 cells are more sensitive to the miR-7 treatment than the poorly invasive MCF-7 cells. Treatment with miR-7 downregulated the expression of EGFR, IGF1R and Wave3 in MDA-MB-231 cells but not in MCF-7 cells. In addition, we further demonstrated that miR-7 inhibited the proliferation, migration and invasion of endothelial cells. And more importantly, miR-7 suppressed the homing and migration of endothelial cells to more aggressive tumour cell conditions. CONCLUSIONS: Given the dual inhibitory effect of miR-7 on metastatic breast cancer cells alone and the interaction of endothelial cells with the tumour-conditioned microenvironment, we suggest miR-7 may be a new therapeutic candidate for its capacity not only to prevent breast cancer cell spreading but also to inhibit tumour-associated angiogenesis in the metastatic breast cancer. |
format | Online Article Text |
id | pubmed-5563947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-55639472018-06-27 MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells Cui, Yu-Xin Bradbury, Robyn Flamini, Valentina Wu, Bo Jordan, Nicola Jiang, Wen G Br J Cancer Molecular Diagnostics BACKGROUND: MicroRNA-7 (miR-7) has been observed as a potent tumour suppressor in multiple cancer types including breast cancer. The aim of this study was to investigate the response sensitivities of metastatic breast cancer cells to miR-7 and the roles of miR-7 in the interaction of endothelial cells and metastatic cancer cells. METHODS: Expression profile of miRNAs in a breast cancer specimen cohort and breast cancer cells were determined using real-time quantitative miRNA assays. Effect of the altering expression of miR-7 on migration, invasion, proliferation, interaction and underlying molecular mechanism of breast cancer cells and endothelial cells was investigated after treatment with the synthesised mimic of miR-7. Luciferase activity analysis was performed to validate Wave-3 as a novel target of miR-7. RESULTS: miR-7 expression was negatively correlated with the stage, grade and survival of the breast cancer patients. There was also differential expression of miRNAs including miR-7 in the breast cancer cells. The synthesised mimic of miR-7 inhibits the motility and wound healing potential of breast cancer cells. The highly metastatic MDA-MB-231 cells are more sensitive to the miR-7 treatment than the poorly invasive MCF-7 cells. Treatment with miR-7 downregulated the expression of EGFR, IGF1R and Wave3 in MDA-MB-231 cells but not in MCF-7 cells. In addition, we further demonstrated that miR-7 inhibited the proliferation, migration and invasion of endothelial cells. And more importantly, miR-7 suppressed the homing and migration of endothelial cells to more aggressive tumour cell conditions. CONCLUSIONS: Given the dual inhibitory effect of miR-7 on metastatic breast cancer cells alone and the interaction of endothelial cells with the tumour-conditioned microenvironment, we suggest miR-7 may be a new therapeutic candidate for its capacity not only to prevent breast cancer cell spreading but also to inhibit tumour-associated angiogenesis in the metastatic breast cancer. Nature Publishing Group 2017-06-27 2017-06-01 /pmc/articles/PMC5563947/ /pubmed/28571043 http://dx.doi.org/10.1038/bjc.2017.156 Text en Copyright © 2017 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Molecular Diagnostics Cui, Yu-Xin Bradbury, Robyn Flamini, Valentina Wu, Bo Jordan, Nicola Jiang, Wen G MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title | MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title_full | MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title_fullStr | MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title_full_unstemmed | MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title_short | MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
title_sort | microrna-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563947/ https://www.ncbi.nlm.nih.gov/pubmed/28571043 http://dx.doi.org/10.1038/bjc.2017.156 |
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