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Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli

The growing use of carbon nanotubes (CNTs) in industrial and consumer products raises important questions about their environmental fate and impact on prokaryotes. In the environment, CNTs are exposed to a variety of conditions (e.g., UV light) that could lead to decomposition and changes in their c...

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Autores principales: Twibell, Brittany, Somerville, Kalie, Manani, Geoffrey, Duszynski, Molly, Wanekaya, Adam, Schweiger, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564384/
https://www.ncbi.nlm.nih.gov/pubmed/28828284
http://dx.doi.org/10.7717/peerj.3721
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author Twibell, Brittany
Somerville, Kalie
Manani, Geoffrey
Duszynski, Molly
Wanekaya, Adam
Schweiger, Paul
author_facet Twibell, Brittany
Somerville, Kalie
Manani, Geoffrey
Duszynski, Molly
Wanekaya, Adam
Schweiger, Paul
author_sort Twibell, Brittany
collection PubMed
description The growing use of carbon nanotubes (CNTs) in industrial and consumer products raises important questions about their environmental fate and impact on prokaryotes. In the environment, CNTs are exposed to a variety of conditions (e.g., UV light) that could lead to decomposition and changes in their chemical properties. Therefore, the potential cytotoxic effect of both pristine and artificially aged carboxyl functionalized CNTRENE(®) C100LM CNTmaterial at neutral and acidic conditions on Escherichia coli K12 was analyzed using a minimal inhibitory concentration (MIC) assay, which also allowed monitoring of non-lethal growth effects. However, there were no observable MIC or significant changes in growth behavior in E. coli K12 when exposed to pristine or aged CNTs. Exposure to pristine CNTRENE(®) C100LM CNT material did not appear to influence cell morphology or damage the cells when examined by electron microscopy. In addition, RNA sequencing revealed no observable regulatory changes in typical stress response pathways. This is surprising considering that previous studies have claimed high cytotoxicity of CNTs, including carboxyl functionalized single-walled CNTs, and suggest that other factors such as trace heavy metals or other impurities are likely responsible for many of the previously reported cytotoxicity in E. coli and possibly other microorganisms.
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spelling pubmed-55643842017-08-21 Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli Twibell, Brittany Somerville, Kalie Manani, Geoffrey Duszynski, Molly Wanekaya, Adam Schweiger, Paul PeerJ Microbiology The growing use of carbon nanotubes (CNTs) in industrial and consumer products raises important questions about their environmental fate and impact on prokaryotes. In the environment, CNTs are exposed to a variety of conditions (e.g., UV light) that could lead to decomposition and changes in their chemical properties. Therefore, the potential cytotoxic effect of both pristine and artificially aged carboxyl functionalized CNTRENE(®) C100LM CNTmaterial at neutral and acidic conditions on Escherichia coli K12 was analyzed using a minimal inhibitory concentration (MIC) assay, which also allowed monitoring of non-lethal growth effects. However, there were no observable MIC or significant changes in growth behavior in E. coli K12 when exposed to pristine or aged CNTs. Exposure to pristine CNTRENE(®) C100LM CNT material did not appear to influence cell morphology or damage the cells when examined by electron microscopy. In addition, RNA sequencing revealed no observable regulatory changes in typical stress response pathways. This is surprising considering that previous studies have claimed high cytotoxicity of CNTs, including carboxyl functionalized single-walled CNTs, and suggest that other factors such as trace heavy metals or other impurities are likely responsible for many of the previously reported cytotoxicity in E. coli and possibly other microorganisms. PeerJ Inc. 2017-08-18 /pmc/articles/PMC5564384/ /pubmed/28828284 http://dx.doi.org/10.7717/peerj.3721 Text en ©2017 Twibell et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Twibell, Brittany
Somerville, Kalie
Manani, Geoffrey
Duszynski, Molly
Wanekaya, Adam
Schweiger, Paul
Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title_full Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title_fullStr Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title_full_unstemmed Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title_short Influence of CNTRENE(®) C100LM carbon nanotube material on the growth and regulation of Escherichia coli
title_sort influence of cntrene(®) c100lm carbon nanotube material on the growth and regulation of escherichia coli
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564384/
https://www.ncbi.nlm.nih.gov/pubmed/28828284
http://dx.doi.org/10.7717/peerj.3721
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