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Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors

The nucleocytoplasmic shuttling of SOX transcription factors play a crucial role in the regulation of SOX protein functions during development. In this study, we have demonstrated two nuclear localization signals in the HMG box of Eriocheir sinensis SOX14A and SOX14B. These two conserved nuclear loc...

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Autores principales: She, Zhen-Yu, Yang, Wan-Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564536/
https://www.ncbi.nlm.nih.gov/pubmed/28187450
http://dx.doi.org/10.18632/oncotarget.15134
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author She, Zhen-Yu
Yang, Wan-Xi
author_facet She, Zhen-Yu
Yang, Wan-Xi
author_sort She, Zhen-Yu
collection PubMed
description The nucleocytoplasmic shuttling of SOX transcription factors play a crucial role in the regulation of SOX protein functions during development. In this study, we have demonstrated two nuclear localization signals in the HMG box of Eriocheir sinensis SOX14A and SOX14B. These two conserved nuclear localization signals mediate nuclear transport. The N-termini nuclear localization signal mediates the calmodulin-dependent pathway and the C-termini nuclear localization signal interacts with the importin-β pathway. The targeted deletion of nuclear localization signals of SOX14A/B dramatically inhibits the nuclear accumulation. We have first time revealed a non-classic nuclear export signal in the HMG box of E. sinensis SOX14A/B proteins is responds to leptomycin B. E. sinensis SOX14A/B is transported from the nucleus to the cytoplasm via a CRM1-dependent nuclear export pathway. And E. sinensis SOX14A/B are not belong to the subgroup E SOX proteins. Furthermore, these findings could shed a light on the mechanisms involved in the nuclear export of SOX proteins. The imperfect nuclear export signal on other SOX proteins, rather than just those of the SOXE group, may also be functional for nuclear export.
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spelling pubmed-55645362017-08-23 Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors She, Zhen-Yu Yang, Wan-Xi Oncotarget Research Paper The nucleocytoplasmic shuttling of SOX transcription factors play a crucial role in the regulation of SOX protein functions during development. In this study, we have demonstrated two nuclear localization signals in the HMG box of Eriocheir sinensis SOX14A and SOX14B. These two conserved nuclear localization signals mediate nuclear transport. The N-termini nuclear localization signal mediates the calmodulin-dependent pathway and the C-termini nuclear localization signal interacts with the importin-β pathway. The targeted deletion of nuclear localization signals of SOX14A/B dramatically inhibits the nuclear accumulation. We have first time revealed a non-classic nuclear export signal in the HMG box of E. sinensis SOX14A/B proteins is responds to leptomycin B. E. sinensis SOX14A/B is transported from the nucleus to the cytoplasm via a CRM1-dependent nuclear export pathway. And E. sinensis SOX14A/B are not belong to the subgroup E SOX proteins. Furthermore, these findings could shed a light on the mechanisms involved in the nuclear export of SOX proteins. The imperfect nuclear export signal on other SOX proteins, rather than just those of the SOXE group, may also be functional for nuclear export. Impact Journals LLC 2017-02-07 /pmc/articles/PMC5564536/ /pubmed/28187450 http://dx.doi.org/10.18632/oncotarget.15134 Text en Copyright: © 2017 She and Yang http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
She, Zhen-Yu
Yang, Wan-Xi
Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title_full Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title_fullStr Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title_full_unstemmed Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title_short Nucleocytoplasmic shuttling of SOX14A and SOX14B transcription factors
title_sort nucleocytoplasmic shuttling of sox14a and sox14b transcription factors
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564536/
https://www.ncbi.nlm.nih.gov/pubmed/28187450
http://dx.doi.org/10.18632/oncotarget.15134
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