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Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression

Transforming growth factor-β1 is considered a key contributor to the progression of breast cancer. MicroRNAs are important factors in the development and progression of many malignancies. In the present study, upon studies of breast cancer cell lines and tissues, we showed that microRNA -196a-3p is...

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Autores principales: Chen, Yan, Huang, Shai, Wu, Bo, Fang, Jiankai, Zhu, Minsheng, Sun, Li, Zhang, Lifeng, Zhang, Yongsheng, Sun, Maomin, Guo, Lingling, Wang, Shouli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564753/
https://www.ncbi.nlm.nih.gov/pubmed/28418877
http://dx.doi.org/10.18632/oncotarget.16308
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author Chen, Yan
Huang, Shai
Wu, Bo
Fang, Jiankai
Zhu, Minsheng
Sun, Li
Zhang, Lifeng
Zhang, Yongsheng
Sun, Maomin
Guo, Lingling
Wang, Shouli
author_facet Chen, Yan
Huang, Shai
Wu, Bo
Fang, Jiankai
Zhu, Minsheng
Sun, Li
Zhang, Lifeng
Zhang, Yongsheng
Sun, Maomin
Guo, Lingling
Wang, Shouli
author_sort Chen, Yan
collection PubMed
description Transforming growth factor-β1 is considered a key contributor to the progression of breast cancer. MicroRNAs are important factors in the development and progression of many malignancies. In the present study, upon studies of breast cancer cell lines and tissues, we showed that microRNA -196a-3p is decreased by transforming growth factor-β1 in breast cancer cells and associated with breast cancer progression. We identified neuropilin-2 as a target gene of microRNA -196a-3p and showed that it is regulated by transforming growth factor-β1. Moreover, transforming growth factor-β1-mediated inhibition of microRNA -196a-3p and activation of neuropilin-2were required for transforming growth factor-β1-induced migration and invasion of breast cancer cells. In addition, neuropilin-2 expression was suppressed in breast tumors, particularly in triple-negative breast cancers. Collectively, our findings strongly indicate that microRNA -196a-3p is a predictive biomarker of breast cancer metastasis and patient survival and a potential therapeutic target in metastatic breast cancer.
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spelling pubmed-55647532017-08-23 Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression Chen, Yan Huang, Shai Wu, Bo Fang, Jiankai Zhu, Minsheng Sun, Li Zhang, Lifeng Zhang, Yongsheng Sun, Maomin Guo, Lingling Wang, Shouli Oncotarget Research Paper Transforming growth factor-β1 is considered a key contributor to the progression of breast cancer. MicroRNAs are important factors in the development and progression of many malignancies. In the present study, upon studies of breast cancer cell lines and tissues, we showed that microRNA -196a-3p is decreased by transforming growth factor-β1 in breast cancer cells and associated with breast cancer progression. We identified neuropilin-2 as a target gene of microRNA -196a-3p and showed that it is regulated by transforming growth factor-β1. Moreover, transforming growth factor-β1-mediated inhibition of microRNA -196a-3p and activation of neuropilin-2were required for transforming growth factor-β1-induced migration and invasion of breast cancer cells. In addition, neuropilin-2 expression was suppressed in breast tumors, particularly in triple-negative breast cancers. Collectively, our findings strongly indicate that microRNA -196a-3p is a predictive biomarker of breast cancer metastasis and patient survival and a potential therapeutic target in metastatic breast cancer. Impact Journals LLC 2017-03-17 /pmc/articles/PMC5564753/ /pubmed/28418877 http://dx.doi.org/10.18632/oncotarget.16308 Text en Copyright: © 2017 Chen et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Chen, Yan
Huang, Shai
Wu, Bo
Fang, Jiankai
Zhu, Minsheng
Sun, Li
Zhang, Lifeng
Zhang, Yongsheng
Sun, Maomin
Guo, Lingling
Wang, Shouli
Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title_full Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title_fullStr Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title_full_unstemmed Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title_short Transforming growth factor-β1 promotes breast cancer metastasis by downregulating miR-196a-3p expression
title_sort transforming growth factor-β1 promotes breast cancer metastasis by downregulating mir-196a-3p expression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564753/
https://www.ncbi.nlm.nih.gov/pubmed/28418877
http://dx.doi.org/10.18632/oncotarget.16308
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