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Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy

BACKGROUND: Adrenocortical carcinoma is a rare finding among common adrenocortical tumors, but it is highly aggressive and requires early detection and treatment. Still, the differential diagnosis between benign and malignant lesions is difficult even for experienced pathologists and there is a sign...

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Autores principales: Koperski, Łukasz, Kotlarek, Marta, Świerniak, Michał, Kolanowska, Monika, Kubiak, Anna, Górnicka, Barbara, Jażdżewski, Krystian, Wójcicka, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564760/
https://www.ncbi.nlm.nih.gov/pubmed/28423361
http://dx.doi.org/10.18632/oncotarget.16788
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author Koperski, Łukasz
Kotlarek, Marta
Świerniak, Michał
Kolanowska, Monika
Kubiak, Anna
Górnicka, Barbara
Jażdżewski, Krystian
Wójcicka, Anna
author_facet Koperski, Łukasz
Kotlarek, Marta
Świerniak, Michał
Kolanowska, Monika
Kubiak, Anna
Górnicka, Barbara
Jażdżewski, Krystian
Wójcicka, Anna
author_sort Koperski, Łukasz
collection PubMed
description BACKGROUND: Adrenocortical carcinoma is a rare finding among common adrenocortical tumors, but it is highly aggressive and requires early detection and treatment. Still, the differential diagnosis between benign and malignant lesions is difficult even for experienced pathologists and there is a significant need for novel diagnostic methods. In this study we aimed to reveal a complete set of microRNAs expressed in the adrenal gland and to identify easily detectable, stable and objective biomarkers of adrenocortical malignancy. METHODS: We employed next-generation sequencing to analyze microRNA profiles in a unique set of 51 samples, assigned to either a learning dataset including 7 adrenocortical carcinomas (ACCs), 8 adrenocortical adenomas (AAs) and 8 control samples (NAs), or a validation dataset including 8 ACCs, 10 AAs and 10 NAs. The results were validated in real-time Q-PCR. RESULTS: We detected 411 miRNAs expressed in 1763 length isoforms in the examined samples. Fifteen miRNAs differentiate between malignant (ACC) and non-malignant (AA + NA) tissue in the test set of independent samples. Expression levels of 6 microRNAs, miR-503-5p, miR-483-3p, miR-450a-5p, miR-210, miR-483-5p, miR-421, predict sample status (malignancy/non-malignancy) with at least 95% accuracy in both datasets. The best single-gene malignancy marker, miR-483-3p, has been validated by real-time RT PCR. CONCLUSIONS: As a result of the study we propose clinically valid and easily detectable biomarkers of adrenocortical malignancy that may significantly facilitate morphological examination. Since microRNAs can be detected in blood, the study brings tools for development of non-invasive diagnostics of adrenocortical carcinomas.
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spelling pubmed-55647602017-08-23 Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy Koperski, Łukasz Kotlarek, Marta Świerniak, Michał Kolanowska, Monika Kubiak, Anna Górnicka, Barbara Jażdżewski, Krystian Wójcicka, Anna Oncotarget Research Paper BACKGROUND: Adrenocortical carcinoma is a rare finding among common adrenocortical tumors, but it is highly aggressive and requires early detection and treatment. Still, the differential diagnosis between benign and malignant lesions is difficult even for experienced pathologists and there is a significant need for novel diagnostic methods. In this study we aimed to reveal a complete set of microRNAs expressed in the adrenal gland and to identify easily detectable, stable and objective biomarkers of adrenocortical malignancy. METHODS: We employed next-generation sequencing to analyze microRNA profiles in a unique set of 51 samples, assigned to either a learning dataset including 7 adrenocortical carcinomas (ACCs), 8 adrenocortical adenomas (AAs) and 8 control samples (NAs), or a validation dataset including 8 ACCs, 10 AAs and 10 NAs. The results were validated in real-time Q-PCR. RESULTS: We detected 411 miRNAs expressed in 1763 length isoforms in the examined samples. Fifteen miRNAs differentiate between malignant (ACC) and non-malignant (AA + NA) tissue in the test set of independent samples. Expression levels of 6 microRNAs, miR-503-5p, miR-483-3p, miR-450a-5p, miR-210, miR-483-5p, miR-421, predict sample status (malignancy/non-malignancy) with at least 95% accuracy in both datasets. The best single-gene malignancy marker, miR-483-3p, has been validated by real-time RT PCR. CONCLUSIONS: As a result of the study we propose clinically valid and easily detectable biomarkers of adrenocortical malignancy that may significantly facilitate morphological examination. Since microRNAs can be detected in blood, the study brings tools for development of non-invasive diagnostics of adrenocortical carcinomas. Impact Journals LLC 2017-04-03 /pmc/articles/PMC5564760/ /pubmed/28423361 http://dx.doi.org/10.18632/oncotarget.16788 Text en Copyright: © 2017 Koperski et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Koperski, Łukasz
Kotlarek, Marta
Świerniak, Michał
Kolanowska, Monika
Kubiak, Anna
Górnicka, Barbara
Jażdżewski, Krystian
Wójcicka, Anna
Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title_full Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title_fullStr Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title_full_unstemmed Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title_short Next-generation sequencing reveals microRNA markers of adrenocortical tumors malignancy
title_sort next-generation sequencing reveals microrna markers of adrenocortical tumors malignancy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564760/
https://www.ncbi.nlm.nih.gov/pubmed/28423361
http://dx.doi.org/10.18632/oncotarget.16788
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