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EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression

Recent advances in the field of circulating tumor cells (CTC) have shown promise in this liquid biopsy-based prognosis of patient outcome. However, not all of the circulating cells are tumor cells, as evidenced by a lack of tumor-specific markers. The current FDA standard for capturing CTCs (CellSea...

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Autores principales: Satelli, Arun, Batth, Izhar, Brownlee, Zachary, Mitra, Abhishek, Zhou, Shouhao, Noh, Hyangsoon, Rojas, Christina R., Li, Heming, Meng, Qing H., Li, Shulin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564771/
https://www.ncbi.nlm.nih.gov/pubmed/28521303
http://dx.doi.org/10.18632/oncotarget.17632
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author Satelli, Arun
Batth, Izhar
Brownlee, Zachary
Mitra, Abhishek
Zhou, Shouhao
Noh, Hyangsoon
Rojas, Christina R.
Li, Heming
Meng, Qing H.
Li, Shulin
author_facet Satelli, Arun
Batth, Izhar
Brownlee, Zachary
Mitra, Abhishek
Zhou, Shouhao
Noh, Hyangsoon
Rojas, Christina R.
Li, Heming
Meng, Qing H.
Li, Shulin
author_sort Satelli, Arun
collection PubMed
description Recent advances in the field of circulating tumor cells (CTC) have shown promise in this liquid biopsy-based prognosis of patient outcome. However, not all of the circulating cells are tumor cells, as evidenced by a lack of tumor-specific markers. The current FDA standard for capturing CTCs (CellSearch) relies on an epithelial marker and cells captured via CellSearch cannot be considered to have undergone EMT. Therefore, it is difficult to ascertain the presence and relevance of any mesenchymal or EMT-like CTCs. To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma, breast, and colon cancer. Here we studied peripheral blood samples of 48 prostate cancer (PCA) patients including hormone sensitive and castration resistant sub-groups. Blood samples were analyzed for three different properties including our own CSV-based CTC enumeration (using 84-1 mAb against CSV), CellSearch-based epithelial CTC counts, and serum prostate-specific antigen (PSA) quantification. Our data demonstrated that in comparison with CellSearch, the CSV-based method had greater sensitivity and specificity. Further, we observed significantly greater numbers of CTCs in castration resistant patients as measured by our CSV method but not CellSearch. Our data suggests CSV-guided CTC enumeration may hold prognostic value and should be further validated as a possible measurement of PCA progression towards the deadly, androgen-independent form.
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spelling pubmed-55647712017-08-23 EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression Satelli, Arun Batth, Izhar Brownlee, Zachary Mitra, Abhishek Zhou, Shouhao Noh, Hyangsoon Rojas, Christina R. Li, Heming Meng, Qing H. Li, Shulin Oncotarget Research Paper Recent advances in the field of circulating tumor cells (CTC) have shown promise in this liquid biopsy-based prognosis of patient outcome. However, not all of the circulating cells are tumor cells, as evidenced by a lack of tumor-specific markers. The current FDA standard for capturing CTCs (CellSearch) relies on an epithelial marker and cells captured via CellSearch cannot be considered to have undergone EMT. Therefore, it is difficult to ascertain the presence and relevance of any mesenchymal or EMT-like CTCs. To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma, breast, and colon cancer. Here we studied peripheral blood samples of 48 prostate cancer (PCA) patients including hormone sensitive and castration resistant sub-groups. Blood samples were analyzed for three different properties including our own CSV-based CTC enumeration (using 84-1 mAb against CSV), CellSearch-based epithelial CTC counts, and serum prostate-specific antigen (PSA) quantification. Our data demonstrated that in comparison with CellSearch, the CSV-based method had greater sensitivity and specificity. Further, we observed significantly greater numbers of CTCs in castration resistant patients as measured by our CSV method but not CellSearch. Our data suggests CSV-guided CTC enumeration may hold prognostic value and should be further validated as a possible measurement of PCA progression towards the deadly, androgen-independent form. Impact Journals LLC 2017-05-04 /pmc/articles/PMC5564771/ /pubmed/28521303 http://dx.doi.org/10.18632/oncotarget.17632 Text en Copyright: © 2017 Satelli et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Satelli, Arun
Batth, Izhar
Brownlee, Zachary
Mitra, Abhishek
Zhou, Shouhao
Noh, Hyangsoon
Rojas, Christina R.
Li, Heming
Meng, Qing H.
Li, Shulin
EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title_full EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title_fullStr EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title_full_unstemmed EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title_short EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
title_sort emt circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564771/
https://www.ncbi.nlm.nih.gov/pubmed/28521303
http://dx.doi.org/10.18632/oncotarget.17632
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