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Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines

PURPOSE: The present study is to discover a new genes associated with drug resistance development in ovarian cancer. METHODS: We used microarray analysis to determine alterations in the level of expression of genes in cisplatin- (CisPt), doxorubicin- (Dox), topotecan- (Top), and paclitaxel- (Pac) re...

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Autores principales: Januchowski, Radosław, Sterzyńska, Karolina, Zawierucha, Piotr, Ruciński, Marcin, Świerczewska, Monika, Partyka, Małgorzata, Bednarek-Rajewska, Katarzyna, Brązert, Maciej, Nowicki, Michał, Zabel, Maciej, Klejewski, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564819/
https://www.ncbi.nlm.nih.gov/pubmed/28611294
http://dx.doi.org/10.18632/oncotarget.18278
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author Januchowski, Radosław
Sterzyńska, Karolina
Zawierucha, Piotr
Ruciński, Marcin
Świerczewska, Monika
Partyka, Małgorzata
Bednarek-Rajewska, Katarzyna
Brązert, Maciej
Nowicki, Michał
Zabel, Maciej
Klejewski, Andrzej
author_facet Januchowski, Radosław
Sterzyńska, Karolina
Zawierucha, Piotr
Ruciński, Marcin
Świerczewska, Monika
Partyka, Małgorzata
Bednarek-Rajewska, Katarzyna
Brązert, Maciej
Nowicki, Michał
Zabel, Maciej
Klejewski, Andrzej
author_sort Januchowski, Radosław
collection PubMed
description PURPOSE: The present study is to discover a new genes associated with drug resistance development in ovarian cancer. METHODS: We used microarray analysis to determine alterations in the level of expression of genes in cisplatin- (CisPt), doxorubicin- (Dox), topotecan- (Top), and paclitaxel- (Pac) resistant variants of W1 and A2780 ovarian cancer cell lines. Immunohistochemistry assay was used to determine protein expression in ovarian cancer patients. RESULTS: We observed alterations in the expression of 22 genes that were common to all three cell lines that were resistant to the same cytostatic drug. The level of expression of 13 genes was upregulated and that of nine genes was downregulated. In the CisPt-resistant cell line, we observed downregulated expression of ABCC6, BST2, ERAP2 and MCTP1; in the Pac-resistant cell line, we observe upregulated expression of ABCB1, EPHA7 and RUNDC3B and downregulated expression of LIPG, MCTP1, NSBP1, PCDH9, PTPRK and SEMA3A. The expression levels of three genes, ABCB1, ABCB4 and IFI16, were upregulated in the Dox-resistant cell lines. In the Top-resistant cell lines, we observed increased expression levels of ABCG2, HERC5, IFIH1, MYOT, S100A3, SAMD4A, SPP1 and TGFBI and decreased expression levels of MCTP1 and PTPRK. The expression of EPHA7, IFI16, SPP1 and TGFBI was confirmed at protein level in analyzed ovarian cancer patients.. CONCLUSIONS: The expression profiles of the investigated cell lines indicated that new candidate genes are related to the development of resistance to the cytostatic drugs that are used in first- and second-line chemotherapy of ovarian cancer.
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spelling pubmed-55648192017-08-23 Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines Januchowski, Radosław Sterzyńska, Karolina Zawierucha, Piotr Ruciński, Marcin Świerczewska, Monika Partyka, Małgorzata Bednarek-Rajewska, Katarzyna Brązert, Maciej Nowicki, Michał Zabel, Maciej Klejewski, Andrzej Oncotarget Research Paper PURPOSE: The present study is to discover a new genes associated with drug resistance development in ovarian cancer. METHODS: We used microarray analysis to determine alterations in the level of expression of genes in cisplatin- (CisPt), doxorubicin- (Dox), topotecan- (Top), and paclitaxel- (Pac) resistant variants of W1 and A2780 ovarian cancer cell lines. Immunohistochemistry assay was used to determine protein expression in ovarian cancer patients. RESULTS: We observed alterations in the expression of 22 genes that were common to all three cell lines that were resistant to the same cytostatic drug. The level of expression of 13 genes was upregulated and that of nine genes was downregulated. In the CisPt-resistant cell line, we observed downregulated expression of ABCC6, BST2, ERAP2 and MCTP1; in the Pac-resistant cell line, we observe upregulated expression of ABCB1, EPHA7 and RUNDC3B and downregulated expression of LIPG, MCTP1, NSBP1, PCDH9, PTPRK and SEMA3A. The expression levels of three genes, ABCB1, ABCB4 and IFI16, were upregulated in the Dox-resistant cell lines. In the Top-resistant cell lines, we observed increased expression levels of ABCG2, HERC5, IFIH1, MYOT, S100A3, SAMD4A, SPP1 and TGFBI and decreased expression levels of MCTP1 and PTPRK. The expression of EPHA7, IFI16, SPP1 and TGFBI was confirmed at protein level in analyzed ovarian cancer patients.. CONCLUSIONS: The expression profiles of the investigated cell lines indicated that new candidate genes are related to the development of resistance to the cytostatic drugs that are used in first- and second-line chemotherapy of ovarian cancer. Impact Journals LLC 2017-05-29 /pmc/articles/PMC5564819/ /pubmed/28611294 http://dx.doi.org/10.18632/oncotarget.18278 Text en Copyright: © 2017 Januchowski et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Januchowski, Radosław
Sterzyńska, Karolina
Zawierucha, Piotr
Ruciński, Marcin
Świerczewska, Monika
Partyka, Małgorzata
Bednarek-Rajewska, Katarzyna
Brązert, Maciej
Nowicki, Michał
Zabel, Maciej
Klejewski, Andrzej
Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title_full Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title_fullStr Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title_full_unstemmed Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title_short Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
title_sort microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564819/
https://www.ncbi.nlm.nih.gov/pubmed/28611294
http://dx.doi.org/10.18632/oncotarget.18278
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