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Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells

Glioblastoma is the most aggressive type of brain tumor with a very poor prognosis. Therefore, it is always of great importance to explore and develop new potential treatment for glioblastoma. Quercetin, a flavonoid present in a variety of human foods, has been shown to inhibit various tumor cell pr...

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Autores principales: Liu, Yue, Tang, Zhen-Gang, Yang, Jian-Quan, Zhou, Yi, Meng, Ling-Hu, Wang, Heng, Li, Cai-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565384/
https://www.ncbi.nlm.nih.gov/pubmed/28860810
http://dx.doi.org/10.2147/OTT.S136821
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author Liu, Yue
Tang, Zhen-Gang
Yang, Jian-Quan
Zhou, Yi
Meng, Ling-Hu
Wang, Heng
Li, Cai-Li
author_facet Liu, Yue
Tang, Zhen-Gang
Yang, Jian-Quan
Zhou, Yi
Meng, Ling-Hu
Wang, Heng
Li, Cai-Li
author_sort Liu, Yue
collection PubMed
description Glioblastoma is the most aggressive type of brain tumor with a very poor prognosis. Therefore, it is always of great importance to explore and develop new potential treatment for glioblastoma. Quercetin, a flavonoid present in a variety of human foods, has been shown to inhibit various tumor cell proliferation. In this study, we found that treating human glioblastoma U251 cells with 10 μg/mL quercetin for 24 hours, a concentration that was far below the IC(50) (113.65 μg/mL) and at which quercetin failed to inhibit cell proliferation, inhibited cell migration (30%) and cell invasion as examined by wound scratch assay and transwell assay, respectively. We further showed that 10 μg/mL quercetin inhibited cell migration and tube formation of human umbilical vein endothelial cells induced by the conditioned medium derived from U251 cell culture. The inhibitory effect of quercetin on migration and angiogenesis is possibly mediated through the downregulation of protein levels of VEGFA, MMP9, and MMP2 as detected by Western blot. Our findings demonstrated that low concentration of quercetin antagonized glioblastoma cell invasion and angiogenesis in vitro.
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spelling pubmed-55653842017-08-31 Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells Liu, Yue Tang, Zhen-Gang Yang, Jian-Quan Zhou, Yi Meng, Ling-Hu Wang, Heng Li, Cai-Li Onco Targets Ther Original Research Glioblastoma is the most aggressive type of brain tumor with a very poor prognosis. Therefore, it is always of great importance to explore and develop new potential treatment for glioblastoma. Quercetin, a flavonoid present in a variety of human foods, has been shown to inhibit various tumor cell proliferation. In this study, we found that treating human glioblastoma U251 cells with 10 μg/mL quercetin for 24 hours, a concentration that was far below the IC(50) (113.65 μg/mL) and at which quercetin failed to inhibit cell proliferation, inhibited cell migration (30%) and cell invasion as examined by wound scratch assay and transwell assay, respectively. We further showed that 10 μg/mL quercetin inhibited cell migration and tube formation of human umbilical vein endothelial cells induced by the conditioned medium derived from U251 cell culture. The inhibitory effect of quercetin on migration and angiogenesis is possibly mediated through the downregulation of protein levels of VEGFA, MMP9, and MMP2 as detected by Western blot. Our findings demonstrated that low concentration of quercetin antagonized glioblastoma cell invasion and angiogenesis in vitro. Dove Medical Press 2017-08-11 /pmc/articles/PMC5565384/ /pubmed/28860810 http://dx.doi.org/10.2147/OTT.S136821 Text en © 2017 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Liu, Yue
Tang, Zhen-Gang
Yang, Jian-Quan
Zhou, Yi
Meng, Ling-Hu
Wang, Heng
Li, Cai-Li
Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title_full Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title_fullStr Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title_full_unstemmed Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title_short Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells
title_sort low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma u251 cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565384/
https://www.ncbi.nlm.nih.gov/pubmed/28860810
http://dx.doi.org/10.2147/OTT.S136821
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