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AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway

BACKGROUND: A Korean herbal formulation, Ojayeonjonghwan, is used to treat late-onset hypogonadism (LOH) symptoms including erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to treatment for ED. Pharmacological effects o...

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Autores principales: Bae, Woong Jin, Zhu, Guan Qun, Choi, Sae Woong, Jeong, Hyun Cheol, Bashraheel, Fahad, Kim, Su Jin, Kim, Sae Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565624/
http://dx.doi.org/10.21037/tau.2017.s095
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author Bae, Woong Jin
Zhu, Guan Qun
Choi, Sae Woong
Jeong, Hyun Cheol
Bashraheel, Fahad
Kim, Su Jin
Kim, Sae Woong
author_facet Bae, Woong Jin
Zhu, Guan Qun
Choi, Sae Woong
Jeong, Hyun Cheol
Bashraheel, Fahad
Kim, Su Jin
Kim, Sae Woong
author_sort Bae, Woong Jin
collection PubMed
description BACKGROUND: A Korean herbal formulation, Ojayeonjonghwan, is used to treat late-onset hypogonadism (LOH) symptoms including erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to treatment for ED. Pharmacological effects of KH-204 were examined in different conditions, including in vitro and vivo (an androgen-deprived rat model). METHODS: We measured the survival rate of TM3 Leydig cells treated by KH-204 under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce the androgen-deprivation. We removed and weighed the testes and epididymides, which would be used to histopathological examination, from the rats in experimental and control groups. Furthermore, we also measured serum testosterone levels, oxidative stress and apoptosis. RESULTS: The results of treated by KH-204: (I) preserved TM3 cells from oxidative stress by improving the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1); (II) lowered the expression of Transforming growth factor-beta (TGF-β) 1/SMAD; (III) increased the average of serum testosterone in androgen-deprived male rats; (IV) kept the activation of spermatogenesis; (V) upgraded contents of 8-hydroxy-20-deoxyguanosine (8-OHdG) and degraded contents of superoxide dismutase (SOD); and (VI) meaningfully reduced apoptosis. CONCLUSIONS: We studied the efficacy of KH-204 as an alternative therapy to improve testicular dysfunction in LOH. The efficacies of KH-204 are likely, at least in part, to degrade oxidative stress through the Nrf2/HO-1 pathway. These findings may offer credible evidences for the use of new complementary and alternative therapies to treat LOH.
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spelling pubmed-55656242017-09-01 AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway Bae, Woong Jin Zhu, Guan Qun Choi, Sae Woong Jeong, Hyun Cheol Bashraheel, Fahad Kim, Su Jin Kim, Sae Woong Transl Androl Urol Printed Abstracts BACKGROUND: A Korean herbal formulation, Ojayeonjonghwan, is used to treat late-onset hypogonadism (LOH) symptoms including erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to treatment for ED. Pharmacological effects of KH-204 were examined in different conditions, including in vitro and vivo (an androgen-deprived rat model). METHODS: We measured the survival rate of TM3 Leydig cells treated by KH-204 under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce the androgen-deprivation. We removed and weighed the testes and epididymides, which would be used to histopathological examination, from the rats in experimental and control groups. Furthermore, we also measured serum testosterone levels, oxidative stress and apoptosis. RESULTS: The results of treated by KH-204: (I) preserved TM3 cells from oxidative stress by improving the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1); (II) lowered the expression of Transforming growth factor-beta (TGF-β) 1/SMAD; (III) increased the average of serum testosterone in androgen-deprived male rats; (IV) kept the activation of spermatogenesis; (V) upgraded contents of 8-hydroxy-20-deoxyguanosine (8-OHdG) and degraded contents of superoxide dismutase (SOD); and (VI) meaningfully reduced apoptosis. CONCLUSIONS: We studied the efficacy of KH-204 as an alternative therapy to improve testicular dysfunction in LOH. The efficacies of KH-204 are likely, at least in part, to degrade oxidative stress through the Nrf2/HO-1 pathway. These findings may offer credible evidences for the use of new complementary and alternative therapies to treat LOH. AME Publishing Company 2017-08 /pmc/articles/PMC5565624/ http://dx.doi.org/10.21037/tau.2017.s095 Text en 2017 Translational Andrology and Urology. All rights reserved.
spellingShingle Printed Abstracts
Bae, Woong Jin
Zhu, Guan Qun
Choi, Sae Woong
Jeong, Hyun Cheol
Bashraheel, Fahad
Kim, Su Jin
Kim, Sae Woong
AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title_full AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title_fullStr AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title_full_unstemmed AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title_short AB095. Antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via Nrf2/HO-1 signalling pathway
title_sort ab095. antioxidant and antifibrotic effect of a herbal formulation in vitro and in the experimental andropause via nrf2/ho-1 signalling pathway
topic Printed Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565624/
http://dx.doi.org/10.21037/tau.2017.s095
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