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Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract

Allium affine is a member of Amaryllidaceae family, which grows wildly in some western regions of Iran. Limited information is available about the pharmacological activities of this plant. The present study aimed to evaluate the fibrinolytic and antioxidant effects of hydroalcoholic extract of A. af...

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Autores principales: Sadeghi, Masoud, Safaeian, Leila, Ghazvini, Mohammadreza Aghaye, Ramezani, Mojtaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566004/
https://www.ncbi.nlm.nih.gov/pubmed/28855941
http://dx.doi.org/10.4103/1735-5362.212047
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author Sadeghi, Masoud
Safaeian, Leila
Ghazvini, Mohammadreza Aghaye
Ramezani, Mojtaba
author_facet Sadeghi, Masoud
Safaeian, Leila
Ghazvini, Mohammadreza Aghaye
Ramezani, Mojtaba
author_sort Sadeghi, Masoud
collection PubMed
description Allium affine is a member of Amaryllidaceae family, which grows wildly in some western regions of Iran. Limited information is available about the pharmacological activities of this plant. The present study aimed to evaluate the fibrinolytic and antioxidant effects of hydroalcoholic extract of A. affine aerial parts. The in vitro antioxidant properties of the extract were evaluated by total phenolic content assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP) assay. The in vivo studies included the determination of hydroperoxides level and FRAP value in serum samples of rats receiving i.p. injections of the plant extract for 21 days. The fibrinolytic activity of the extract was quantitatively evaluated by measuring the clot weight. In vitro antioxidant analysis exhibited the promising potential of DPPH scavenging and total antioxidant capacity of A. affine extract. In the in vivo analysis, A. affine extract reduced the serum hydroperoxides level and increased the serum total antioxidant capacity in rats. In vitro fibrinolytic assay also elucidated notable thrombolytic activity of the plant extract. The results of this study revealed the valuable antioxidant and in vitro fibrinolytic activities of A. affine extract. Further studies are needed for better evaluation of anticoagulant and thrombolytic activities of this plant and understanding its detailed mechanisms.
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spelling pubmed-55660042017-08-30 Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract Sadeghi, Masoud Safaeian, Leila Ghazvini, Mohammadreza Aghaye Ramezani, Mojtaba Res Pharm Sci Original Article Allium affine is a member of Amaryllidaceae family, which grows wildly in some western regions of Iran. Limited information is available about the pharmacological activities of this plant. The present study aimed to evaluate the fibrinolytic and antioxidant effects of hydroalcoholic extract of A. affine aerial parts. The in vitro antioxidant properties of the extract were evaluated by total phenolic content assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP) assay. The in vivo studies included the determination of hydroperoxides level and FRAP value in serum samples of rats receiving i.p. injections of the plant extract for 21 days. The fibrinolytic activity of the extract was quantitatively evaluated by measuring the clot weight. In vitro antioxidant analysis exhibited the promising potential of DPPH scavenging and total antioxidant capacity of A. affine extract. In the in vivo analysis, A. affine extract reduced the serum hydroperoxides level and increased the serum total antioxidant capacity in rats. In vitro fibrinolytic assay also elucidated notable thrombolytic activity of the plant extract. The results of this study revealed the valuable antioxidant and in vitro fibrinolytic activities of A. affine extract. Further studies are needed for better evaluation of anticoagulant and thrombolytic activities of this plant and understanding its detailed mechanisms. Medknow Publications & Media Pvt Ltd 2017-08 /pmc/articles/PMC5566004/ /pubmed/28855941 http://dx.doi.org/10.4103/1735-5362.212047 Text en Copyright: © 2017 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sadeghi, Masoud
Safaeian, Leila
Ghazvini, Mohammadreza Aghaye
Ramezani, Mojtaba
Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title_full Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title_fullStr Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title_full_unstemmed Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title_short Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract
title_sort evaluation of fibrinolytic and antioxidant effects of allium affine hydroalcoholic extract
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566004/
https://www.ncbi.nlm.nih.gov/pubmed/28855941
http://dx.doi.org/10.4103/1735-5362.212047
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