The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication

The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter. With an anti-HBV pri-miR31 mimic integrated between two HBV-specific gRNAs,...

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Detalles Bibliográficos
Autores principales: Wang, Jie, Chen, Ran, Zhang, Ruiyang, Ding, Shanlong, Zhang, Tianying, Yuan, Quan, Guan, Guiwen, Chen, Xiangmei, Zhang, Ting, Zhuang, Hui, Nunes, Frederick, Block, Timothy, Liu, Shuang, Duan, Zhongping, Xia, Ningshao, Xu, Zhongwei, Lu, Fengmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566108/
https://www.ncbi.nlm.nih.gov/pubmed/28839466
http://dx.doi.org/10.7150/thno.18114

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