Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria

Nasopharyngeal myiases are caused by larvae of bot flies (Diptera: Oestridae), which have evolved a high specificity for their hosts. Bot flies (n = 916) were collected from 137 (57.6 %) out of 238 red deer (Cervus elaphus) hunted in Vorarlberg and Tyrol (Western Austria). After being stored in 75 %...

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Autores principales: Leitner, Natascha, Schwarzmann, Laurin, Zittra, Carina, Palmieri, Nicola, Eigner, Barbara, Otranto, Domenico, Glawischnig, Walter, Fuehrer, Hans-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566181/
https://www.ncbi.nlm.nih.gov/pubmed/27492199
http://dx.doi.org/10.1007/s00436-016-5206-z
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author Leitner, Natascha
Schwarzmann, Laurin
Zittra, Carina
Palmieri, Nicola
Eigner, Barbara
Otranto, Domenico
Glawischnig, Walter
Fuehrer, Hans-Peter
author_facet Leitner, Natascha
Schwarzmann, Laurin
Zittra, Carina
Palmieri, Nicola
Eigner, Barbara
Otranto, Domenico
Glawischnig, Walter
Fuehrer, Hans-Peter
author_sort Leitner, Natascha
collection PubMed
description Nasopharyngeal myiases are caused by larvae of bot flies (Diptera: Oestridae), which have evolved a high specificity for their hosts. Bot flies (n = 916) were collected from 137 (57.6 %) out of 238 red deer (Cervus elaphus) hunted in Vorarlberg and Tyrol (Western Austria). After being stored in 75 % ethanol, larvae were identified to species level and developmental stage using morphological and morphometric keys. Larvae were also molecularly characterized by polymerase chain reaction (PCR) amplification and partial sequencing of the mitochondrial cytochrome oxidase subunit I gene. Morphological and molecular analysis allowed identification of larvae as Cephenemyia auribarbis and Pharyngomyia picta. Genetic variations were also examined within the specimens collected in both geographical locations.
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spelling pubmed-55661812017-09-19 Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria Leitner, Natascha Schwarzmann, Laurin Zittra, Carina Palmieri, Nicola Eigner, Barbara Otranto, Domenico Glawischnig, Walter Fuehrer, Hans-Peter Parasitol Res Short Communication Nasopharyngeal myiases are caused by larvae of bot flies (Diptera: Oestridae), which have evolved a high specificity for their hosts. Bot flies (n = 916) were collected from 137 (57.6 %) out of 238 red deer (Cervus elaphus) hunted in Vorarlberg and Tyrol (Western Austria). After being stored in 75 % ethanol, larvae were identified to species level and developmental stage using morphological and morphometric keys. Larvae were also molecularly characterized by polymerase chain reaction (PCR) amplification and partial sequencing of the mitochondrial cytochrome oxidase subunit I gene. Morphological and molecular analysis allowed identification of larvae as Cephenemyia auribarbis and Pharyngomyia picta. Genetic variations were also examined within the specimens collected in both geographical locations. Springer Berlin Heidelberg 2016-08-05 2016 /pmc/articles/PMC5566181/ /pubmed/27492199 http://dx.doi.org/10.1007/s00436-016-5206-z Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Communication
Leitner, Natascha
Schwarzmann, Laurin
Zittra, Carina
Palmieri, Nicola
Eigner, Barbara
Otranto, Domenico
Glawischnig, Walter
Fuehrer, Hans-Peter
Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title_full Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title_fullStr Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title_full_unstemmed Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title_short Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria
title_sort morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (cervus elaphus) in austria
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566181/
https://www.ncbi.nlm.nih.gov/pubmed/27492199
http://dx.doi.org/10.1007/s00436-016-5206-z
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