Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform

A sensitive turn-on luminescent sensor for H(2)O(2) based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H(2)O(2), the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. Howeve...

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Autores principales: Liu, Jinshui, Dong, Zhen-Zhen, Yang, Chao, Li, Guodong, Wu, Chun, Lee, Fu-Wa, Leung, Chung-Hang, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566206/
https://www.ncbi.nlm.nih.gov/pubmed/28827747
http://dx.doi.org/10.1038/s41598-017-09478-6
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author Liu, Jinshui
Dong, Zhen-Zhen
Yang, Chao
Li, Guodong
Wu, Chun
Lee, Fu-Wa
Leung, Chung-Hang
Ma, Dik-Lung
author_facet Liu, Jinshui
Dong, Zhen-Zhen
Yang, Chao
Li, Guodong
Wu, Chun
Lee, Fu-Wa
Leung, Chung-Hang
Ma, Dik-Lung
author_sort Liu, Jinshui
collection PubMed
description A sensitive turn-on luminescent sensor for H(2)O(2) based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H(2)O(2), the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H(2)O(2) can oxidize AgNPs to soluble Ag(+) cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H(2)O(2) in the range of 0−17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H(2)O(2) in living cells, and it also showed high selectivity for H(2)O(2) over other interfering substances.
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spelling pubmed-55662062017-08-23 Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform Liu, Jinshui Dong, Zhen-Zhen Yang, Chao Li, Guodong Wu, Chun Lee, Fu-Wa Leung, Chung-Hang Ma, Dik-Lung Sci Rep Article A sensitive turn-on luminescent sensor for H(2)O(2) based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H(2)O(2), the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H(2)O(2) can oxidize AgNPs to soluble Ag(+) cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H(2)O(2) in the range of 0−17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H(2)O(2) in living cells, and it also showed high selectivity for H(2)O(2) over other interfering substances. Nature Publishing Group UK 2017-08-21 /pmc/articles/PMC5566206/ /pubmed/28827747 http://dx.doi.org/10.1038/s41598-017-09478-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Jinshui
Dong, Zhen-Zhen
Yang, Chao
Li, Guodong
Wu, Chun
Lee, Fu-Wa
Leung, Chung-Hang
Ma, Dik-Lung
Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title_full Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title_fullStr Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title_full_unstemmed Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title_short Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform
title_sort turn-on luminescent probe for hydrogen peroxide sensing and imaging in living cells based on an iridium(iii) complex–silver nanoparticle platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566206/
https://www.ncbi.nlm.nih.gov/pubmed/28827747
http://dx.doi.org/10.1038/s41598-017-09478-6
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