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Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze elec...

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Autores principales: Marshall, Christopher W., Ross, Daniel E., Handley, Kim M., Weisenhorn, Pamela B., Edirisinghe, Janaka N., Henry, Christopher S., Gilbert, Jack A., May, Harold D., Norman, R. Sean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566340/
https://www.ncbi.nlm.nih.gov/pubmed/28827682
http://dx.doi.org/10.1038/s41598-017-08877-z
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author Marshall, Christopher W.
Ross, Daniel E.
Handley, Kim M.
Weisenhorn, Pamela B.
Edirisinghe, Janaka N.
Henry, Christopher S.
Gilbert, Jack A.
May, Harold D.
Norman, R. Sean
author_facet Marshall, Christopher W.
Ross, Daniel E.
Handley, Kim M.
Weisenhorn, Pamela B.
Edirisinghe, Janaka N.
Henry, Christopher S.
Gilbert, Jack A.
May, Harold D.
Norman, R. Sean
author_sort Marshall, Christopher W.
collection PubMed
description Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. We assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. These molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.
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spelling pubmed-55663402017-08-23 Metabolic Reconstruction and Modeling Microbial Electrosynthesis Marshall, Christopher W. Ross, Daniel E. Handley, Kim M. Weisenhorn, Pamela B. Edirisinghe, Janaka N. Henry, Christopher S. Gilbert, Jack A. May, Harold D. Norman, R. Sean Sci Rep Article Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. We assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. These molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities. Nature Publishing Group UK 2017-08-21 /pmc/articles/PMC5566340/ /pubmed/28827682 http://dx.doi.org/10.1038/s41598-017-08877-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marshall, Christopher W.
Ross, Daniel E.
Handley, Kim M.
Weisenhorn, Pamela B.
Edirisinghe, Janaka N.
Henry, Christopher S.
Gilbert, Jack A.
May, Harold D.
Norman, R. Sean
Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_full Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_fullStr Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_full_unstemmed Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_short Metabolic Reconstruction and Modeling Microbial Electrosynthesis
title_sort metabolic reconstruction and modeling microbial electrosynthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566340/
https://www.ncbi.nlm.nih.gov/pubmed/28827682
http://dx.doi.org/10.1038/s41598-017-08877-z
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