Cargando…

Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery

Obesity is characterized by insulin-resistance (IR), enhanced lipolysis, and ectopic, inflamed fat. We related the histology of subcutaneous (SAT), visceral fat (VAT), and skeletal muscle to the metabolic abnormalities, and tested their mutual changes after bariatric surgery in type 2 diabetic (T2D)...

Descripción completa

Detalles Bibliográficos
Autores principales: Camastra, Stefania, Vitali, Alessandra, Anselmino, Marco, Gastaldelli, Amalia, Bellini, Rosario, Berta, Rossana, Severi, Ilenia, Baldi, Simona, Astiarraga, Brenno, Barbatelli, Giorgio, Cinti, Saverio, Ferrannini, Ele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566429/
https://www.ncbi.nlm.nih.gov/pubmed/28827671
http://dx.doi.org/10.1038/s41598-017-08444-6
_version_ 1783258550426927104
author Camastra, Stefania
Vitali, Alessandra
Anselmino, Marco
Gastaldelli, Amalia
Bellini, Rosario
Berta, Rossana
Severi, Ilenia
Baldi, Simona
Astiarraga, Brenno
Barbatelli, Giorgio
Cinti, Saverio
Ferrannini, Ele
author_facet Camastra, Stefania
Vitali, Alessandra
Anselmino, Marco
Gastaldelli, Amalia
Bellini, Rosario
Berta, Rossana
Severi, Ilenia
Baldi, Simona
Astiarraga, Brenno
Barbatelli, Giorgio
Cinti, Saverio
Ferrannini, Ele
author_sort Camastra, Stefania
collection PubMed
description Obesity is characterized by insulin-resistance (IR), enhanced lipolysis, and ectopic, inflamed fat. We related the histology of subcutaneous (SAT), visceral fat (VAT), and skeletal muscle to the metabolic abnormalities, and tested their mutual changes after bariatric surgery in type 2 diabetic (T2D) and weight-matched non-diabetic (ND) patients. We measured IR (insulin clamp), lipolysis ((2)H(5)-glycerol infusion), ß-cell glucose-sensitivity (ß-GS, mathematical modeling), and VAT, SAT, and rectus abdominis histology (light and electron microscopy). Presurgery, SAT and VAT showed signs of fibrosis/necrosis, small mitochondria, free interstitial lipids, thickened capillary basement membrane. Compared to ND, T2D had impaired ß-GS, intracapillary neutrophils and higher intramyocellular fat, adipocyte area in VAT, crown-like structures (CLS) in VAT and SAT with rare structures (cyst-like) ~10-fold larger than CLS. Fat expansion was associated with enhanced lipolysis and IR. VAT histology and intramyocellular fat were related to impaired ß-GS. Postsurgery, IR and lipolysis improved in all, ß-GS improved in T2D. Muscle fat infiltration was reduced, adipocytes were smaller and richer in mitochondria, and CLS density in SAT was reduced. In conclusion, IR improves proportionally to weight loss but remains subnormal, whilst SAT and muscle changes disappear. In T2D postsurgery, some VAT pathology persists and beta-cell dysfunction improves but is not normalized.
format Online
Article
Text
id pubmed-5566429
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55664292017-08-23 Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery Camastra, Stefania Vitali, Alessandra Anselmino, Marco Gastaldelli, Amalia Bellini, Rosario Berta, Rossana Severi, Ilenia Baldi, Simona Astiarraga, Brenno Barbatelli, Giorgio Cinti, Saverio Ferrannini, Ele Sci Rep Article Obesity is characterized by insulin-resistance (IR), enhanced lipolysis, and ectopic, inflamed fat. We related the histology of subcutaneous (SAT), visceral fat (VAT), and skeletal muscle to the metabolic abnormalities, and tested their mutual changes after bariatric surgery in type 2 diabetic (T2D) and weight-matched non-diabetic (ND) patients. We measured IR (insulin clamp), lipolysis ((2)H(5)-glycerol infusion), ß-cell glucose-sensitivity (ß-GS, mathematical modeling), and VAT, SAT, and rectus abdominis histology (light and electron microscopy). Presurgery, SAT and VAT showed signs of fibrosis/necrosis, small mitochondria, free interstitial lipids, thickened capillary basement membrane. Compared to ND, T2D had impaired ß-GS, intracapillary neutrophils and higher intramyocellular fat, adipocyte area in VAT, crown-like structures (CLS) in VAT and SAT with rare structures (cyst-like) ~10-fold larger than CLS. Fat expansion was associated with enhanced lipolysis and IR. VAT histology and intramyocellular fat were related to impaired ß-GS. Postsurgery, IR and lipolysis improved in all, ß-GS improved in T2D. Muscle fat infiltration was reduced, adipocytes were smaller and richer in mitochondria, and CLS density in SAT was reduced. In conclusion, IR improves proportionally to weight loss but remains subnormal, whilst SAT and muscle changes disappear. In T2D postsurgery, some VAT pathology persists and beta-cell dysfunction improves but is not normalized. Nature Publishing Group UK 2017-08-21 /pmc/articles/PMC5566429/ /pubmed/28827671 http://dx.doi.org/10.1038/s41598-017-08444-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Camastra, Stefania
Vitali, Alessandra
Anselmino, Marco
Gastaldelli, Amalia
Bellini, Rosario
Berta, Rossana
Severi, Ilenia
Baldi, Simona
Astiarraga, Brenno
Barbatelli, Giorgio
Cinti, Saverio
Ferrannini, Ele
Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title_full Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title_fullStr Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title_full_unstemmed Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title_short Muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
title_sort muscle and adipose tissue morphology, insulin sensitivity and beta-cell function in diabetic and nondiabetic obese patients: effects of bariatric surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566429/
https://www.ncbi.nlm.nih.gov/pubmed/28827671
http://dx.doi.org/10.1038/s41598-017-08444-6
work_keys_str_mv AT camastrastefania muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT vitalialessandra muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT anselminomarco muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT gastaldelliamalia muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT bellinirosario muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT bertarossana muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT severiilenia muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT baldisimona muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT astiarragabrenno muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT barbatelligiorgio muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT cintisaverio muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery
AT ferranniniele muscleandadiposetissuemorphologyinsulinsensitivityandbetacellfunctionindiabeticandnondiabeticobesepatientseffectsofbariatricsurgery