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Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints

Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rate—even on line—to purposeful levels higher prod...

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Detalles Bibliográficos
Autores principales: Marschall, Lukas, Sagmeister, Patrick, Herwig, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566544/
https://www.ncbi.nlm.nih.gov/pubmed/27999902
http://dx.doi.org/10.1007/s00253-016-8045-z

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