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Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints
Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rate—even on line—to purposeful levels higher prod...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566544/ https://www.ncbi.nlm.nih.gov/pubmed/27999902 http://dx.doi.org/10.1007/s00253-016-8045-z |