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Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation

Androgen deficiency is strongly associated with erectile dysfunction (ED). Inadequate penile arterial blood flow is one of the major causes of ED. The blood flow to the corpus cavernosum is mainly derived from the internal pudendal arteries (IPAs); however, no study has evaluated the effects of andr...

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Autores principales: Alves-Lopes, Rhéure, Neves, Karla B, Silva, Marcondes AB, Olivon, Vânia C, Ruginsk, Silvia G, Antunes-Rodrigues, José, Ramalho, Leandra NZ, Tostes, Rita C, Carneiro, Fernando Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566844/
https://www.ncbi.nlm.nih.gov/pubmed/27391248
http://dx.doi.org/10.4103/1008-682X.173935
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author Alves-Lopes, Rhéure
Neves, Karla B
Silva, Marcondes AB
Olivon, Vânia C
Ruginsk, Silvia G
Antunes-Rodrigues, José
Ramalho, Leandra NZ
Tostes, Rita C
Carneiro, Fernando Silva
author_facet Alves-Lopes, Rhéure
Neves, Karla B
Silva, Marcondes AB
Olivon, Vânia C
Ruginsk, Silvia G
Antunes-Rodrigues, José
Ramalho, Leandra NZ
Tostes, Rita C
Carneiro, Fernando Silva
author_sort Alves-Lopes, Rhéure
collection PubMed
description Androgen deficiency is strongly associated with erectile dysfunction (ED). Inadequate penile arterial blood flow is one of the major causes of ED. The blood flow to the corpus cavernosum is mainly derived from the internal pudendal arteries (IPAs); however, no study has evaluated the effects of androgen deprivation on IPA's function. We hypothesized that castration impairs IPAs reactivity and structure, contributing to ED. In our study, Wistar male rats, 8-week-old, were castrated and studied 30 days after orchiectomy. Functional and structural properties of rat IPAs were determined using wire and pressure myograph systems, respectively. Protein expression was determined by Western blot and immunohistochemistry. Plasma testosterone levels were determined using the IMMULITE 1000 Immunoassay System. Castrated rats exhibited impaired erectile function, represented by decreased intracavernosal pressure/mean arterial pressure ratio. IPAs from castrated rats exhibited decreased phenylephrine- and electrical field stimulation (EFS)-induced contraction and decreased acetylcholine- and EFS-induced vasodilatation. IPAs from castrated rats exhibited decreased internal diameter, external diameter, thickness of the arterial wall, and cross-sectional area. Castration decreased nNOS and α-actin expression and increased collagen expression, p38 (Thr180/Tyr182) phosphorylation, as well as caspase 3 cleavage. In conclusion, androgen deficiency is associated with impairment of IPA reactivity and structure and increased apoptosis signaling markers. Our findings suggest that androgen deficiency-induced vascular dysfunction is an event involving hypotrophic vascular remodeling of IPAs.
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spelling pubmed-55668442017-09-02 Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation Alves-Lopes, Rhéure Neves, Karla B Silva, Marcondes AB Olivon, Vânia C Ruginsk, Silvia G Antunes-Rodrigues, José Ramalho, Leandra NZ Tostes, Rita C Carneiro, Fernando Silva Asian J Androl Original Article Androgen deficiency is strongly associated with erectile dysfunction (ED). Inadequate penile arterial blood flow is one of the major causes of ED. The blood flow to the corpus cavernosum is mainly derived from the internal pudendal arteries (IPAs); however, no study has evaluated the effects of androgen deprivation on IPA's function. We hypothesized that castration impairs IPAs reactivity and structure, contributing to ED. In our study, Wistar male rats, 8-week-old, were castrated and studied 30 days after orchiectomy. Functional and structural properties of rat IPAs were determined using wire and pressure myograph systems, respectively. Protein expression was determined by Western blot and immunohistochemistry. Plasma testosterone levels were determined using the IMMULITE 1000 Immunoassay System. Castrated rats exhibited impaired erectile function, represented by decreased intracavernosal pressure/mean arterial pressure ratio. IPAs from castrated rats exhibited decreased phenylephrine- and electrical field stimulation (EFS)-induced contraction and decreased acetylcholine- and EFS-induced vasodilatation. IPAs from castrated rats exhibited decreased internal diameter, external diameter, thickness of the arterial wall, and cross-sectional area. Castration decreased nNOS and α-actin expression and increased collagen expression, p38 (Thr180/Tyr182) phosphorylation, as well as caspase 3 cleavage. In conclusion, androgen deficiency is associated with impairment of IPA reactivity and structure and increased apoptosis signaling markers. Our findings suggest that androgen deficiency-induced vascular dysfunction is an event involving hypotrophic vascular remodeling of IPAs. Medknow Publications & Media Pvt Ltd 2017 2016-07-05 /pmc/articles/PMC5566844/ /pubmed/27391248 http://dx.doi.org/10.4103/1008-682X.173935 Text en Copyright: © The Author(s)(2017) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Alves-Lopes, Rhéure
Neves, Karla B
Silva, Marcondes AB
Olivon, Vânia C
Ruginsk, Silvia G
Antunes-Rodrigues, José
Ramalho, Leandra NZ
Tostes, Rita C
Carneiro, Fernando Silva
Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title_full Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title_fullStr Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title_full_unstemmed Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title_short Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
title_sort functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566844/
https://www.ncbi.nlm.nih.gov/pubmed/27391248
http://dx.doi.org/10.4103/1008-682X.173935
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