Cargando…
The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation
SUN1, a component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, functions in mammalian mRNA export through the NXF1-dependent pathway. It associates with mRNP complexes by direct interaction with NXF1. It also binds to the NPC through association with the nuclear pore component Nu...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567243/ https://www.ncbi.nlm.nih.gov/pubmed/28831067 http://dx.doi.org/10.1038/s41598-017-08837-7 |
_version_ | 1783258688079790080 |
---|---|
author | Li, Ping Stumpf, Maria Müller, Rolf Eichinger, Ludwig Glöckner, Gernot Noegel, Angelika A. |
author_facet | Li, Ping Stumpf, Maria Müller, Rolf Eichinger, Ludwig Glöckner, Gernot Noegel, Angelika A. |
author_sort | Li, Ping |
collection | PubMed |
description | SUN1, a component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, functions in mammalian mRNA export through the NXF1-dependent pathway. It associates with mRNP complexes by direct interaction with NXF1. It also binds to the NPC through association with the nuclear pore component Nup153, which is involved in mRNA export. The SUN1-NXF1 association is at least partly regulated by a protein kinase C (PKC) which phosphorylates serine 113 (S113) in the N-terminal domain leading to reduced interaction. The phosphorylation appears to be important for the SUN1 function in nuclear mRNA export since GFP-SUN1 carrying a S113A mutation was less efficient in restoring mRNA export after SUN1 knockdown as compared to the wild type protein. By contrast, GFP-SUN1-S113D resembling the phosphorylated state allowed very efficient export of poly(A)+RNA. Furthermore, probing a possible role of the LINC complex component Nesprin-2 in this process we observed impaired mRNA export in Nesprin-2 knockdown cells. This effect might be independent of SUN1 as expression of a GFP tagged SUN-domain deficient SUN1, which no longer can interact with Nesprin-2, did not affect mRNA export. |
format | Online Article Text |
id | pubmed-5567243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55672432017-09-01 The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation Li, Ping Stumpf, Maria Müller, Rolf Eichinger, Ludwig Glöckner, Gernot Noegel, Angelika A. Sci Rep Article SUN1, a component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, functions in mammalian mRNA export through the NXF1-dependent pathway. It associates with mRNP complexes by direct interaction with NXF1. It also binds to the NPC through association with the nuclear pore component Nup153, which is involved in mRNA export. The SUN1-NXF1 association is at least partly regulated by a protein kinase C (PKC) which phosphorylates serine 113 (S113) in the N-terminal domain leading to reduced interaction. The phosphorylation appears to be important for the SUN1 function in nuclear mRNA export since GFP-SUN1 carrying a S113A mutation was less efficient in restoring mRNA export after SUN1 knockdown as compared to the wild type protein. By contrast, GFP-SUN1-S113D resembling the phosphorylated state allowed very efficient export of poly(A)+RNA. Furthermore, probing a possible role of the LINC complex component Nesprin-2 in this process we observed impaired mRNA export in Nesprin-2 knockdown cells. This effect might be independent of SUN1 as expression of a GFP tagged SUN-domain deficient SUN1, which no longer can interact with Nesprin-2, did not affect mRNA export. Nature Publishing Group UK 2017-08-22 /pmc/articles/PMC5567243/ /pubmed/28831067 http://dx.doi.org/10.1038/s41598-017-08837-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Ping Stumpf, Maria Müller, Rolf Eichinger, Ludwig Glöckner, Gernot Noegel, Angelika A. The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title | The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title_full | The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title_fullStr | The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title_full_unstemmed | The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title_short | The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation |
title_sort | function of the inner nuclear envelope protein sun1 in mrna export is regulated by phosphorylation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567243/ https://www.ncbi.nlm.nih.gov/pubmed/28831067 http://dx.doi.org/10.1038/s41598-017-08837-7 |
work_keys_str_mv | AT liping thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT stumpfmaria thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT mullerrolf thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT eichingerludwig thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT glocknergernot thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT noegelangelikaa thefunctionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT liping functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT stumpfmaria functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT mullerrolf functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT eichingerludwig functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT glocknergernot functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation AT noegelangelikaa functionoftheinnernuclearenvelopeproteinsun1inmrnaexportisregulatedbyphosphorylation |