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Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery

Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine receptors (ACh...

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Autores principales: Bernadzki, Krzysztof M., Gawor, Marta, Pęziński, Marcin, Mazurek, Paula, Niewiadomski, Paweł, Rędowicz, Maria J., Prószyński, Tomasz J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567263/
https://www.ncbi.nlm.nih.gov/pubmed/28831123
http://dx.doi.org/10.1038/s41598-017-09590-7
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author Bernadzki, Krzysztof M.
Gawor, Marta
Pęziński, Marcin
Mazurek, Paula
Niewiadomski, Paweł
Rędowicz, Maria J.
Prószyński, Tomasz J.
author_facet Bernadzki, Krzysztof M.
Gawor, Marta
Pęziński, Marcin
Mazurek, Paula
Niewiadomski, Paweł
Rędowicz, Maria J.
Prószyński, Tomasz J.
author_sort Bernadzki, Krzysztof M.
collection PubMed
description Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine receptors (AChR) are indispensable for proper NMJ function. Abnormalities in the organization of synaptic components often cause severe neuromuscular disorders, such as muscular dystrophy. The dystrophin-associated glycoprotein complex (DGC) was shown to play an important role in NMJ development. We recently identified liprin-α-1 as a novel binding partner for one of the cytoplasmic DGC components, α-dystrobrevin-1. In the present study, we performed a detailed analysis of localization and function of liprin-α-1 at the murine NMJ. We showed that liprin-α-1 localizes to both pre- and postsynaptic compartments at the NMJ, and its synaptic enrichment depends on the presence of the nerve. Using cultured muscle cells, we found that liprin-α-1 plays an important role in AChR clustering and the organization of cortical microtubules. Our studies provide novel insights into the function of liprin-α-1 at vertebrate neuromuscular synapses.
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spelling pubmed-55672632017-09-01 Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery Bernadzki, Krzysztof M. Gawor, Marta Pęziński, Marcin Mazurek, Paula Niewiadomski, Paweł Rędowicz, Maria J. Prószyński, Tomasz J. Sci Rep Article Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine receptors (AChR) are indispensable for proper NMJ function. Abnormalities in the organization of synaptic components often cause severe neuromuscular disorders, such as muscular dystrophy. The dystrophin-associated glycoprotein complex (DGC) was shown to play an important role in NMJ development. We recently identified liprin-α-1 as a novel binding partner for one of the cytoplasmic DGC components, α-dystrobrevin-1. In the present study, we performed a detailed analysis of localization and function of liprin-α-1 at the murine NMJ. We showed that liprin-α-1 localizes to both pre- and postsynaptic compartments at the NMJ, and its synaptic enrichment depends on the presence of the nerve. Using cultured muscle cells, we found that liprin-α-1 plays an important role in AChR clustering and the organization of cortical microtubules. Our studies provide novel insights into the function of liprin-α-1 at vertebrate neuromuscular synapses. Nature Publishing Group UK 2017-08-22 /pmc/articles/PMC5567263/ /pubmed/28831123 http://dx.doi.org/10.1038/s41598-017-09590-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bernadzki, Krzysztof M.
Gawor, Marta
Pęziński, Marcin
Mazurek, Paula
Niewiadomski, Paweł
Rędowicz, Maria J.
Prószyński, Tomasz J.
Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_full Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_fullStr Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_full_unstemmed Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_short Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_sort liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567263/
https://www.ncbi.nlm.nih.gov/pubmed/28831123
http://dx.doi.org/10.1038/s41598-017-09590-7
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