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Stimulation triggers endogenous activity patterns in cultured cortical networks

Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network b...

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Autores principales: Pasquale, Valentina, Martinoia, Sergio, Chiappalone, Michela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567348/
https://www.ncbi.nlm.nih.gov/pubmed/28831071
http://dx.doi.org/10.1038/s41598-017-08369-0
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author Pasquale, Valentina
Martinoia, Sergio
Chiappalone, Michela
author_facet Pasquale, Valentina
Martinoia, Sergio
Chiappalone, Michela
author_sort Pasquale, Valentina
collection PubMed
description Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network bursts) with periods of quiescence in a dynamic state which recalls the fluctuation of in vivo UP and DOWN states. Network bursts can also be elicited by external stimulation and their spatial propagation patterns tracked by means of multi-channel micro-electrode arrays. In this study, we used rat cortical cultures coupled to micro-electrode arrays to investigate the similarity between spontaneous and evoked activity patterns. We performed experiments by applying electrical stimulation to different network locations and demonstrated that the rank orders of electrodes during evoked and spontaneous events are remarkably similar independently from the stimulation source. We linked this result to the capability of stimulation to evoke firing in highly active and “leader” sites of the network, reliably and rapidly recruited within both spontaneous and evoked bursts. Our study provides the first evidence that spontaneous and evoked activity similarity is reliably observed also in dissociated cortical networks.
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spelling pubmed-55673482017-09-01 Stimulation triggers endogenous activity patterns in cultured cortical networks Pasquale, Valentina Martinoia, Sergio Chiappalone, Michela Sci Rep Article Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network bursts) with periods of quiescence in a dynamic state which recalls the fluctuation of in vivo UP and DOWN states. Network bursts can also be elicited by external stimulation and their spatial propagation patterns tracked by means of multi-channel micro-electrode arrays. In this study, we used rat cortical cultures coupled to micro-electrode arrays to investigate the similarity between spontaneous and evoked activity patterns. We performed experiments by applying electrical stimulation to different network locations and demonstrated that the rank orders of electrodes during evoked and spontaneous events are remarkably similar independently from the stimulation source. We linked this result to the capability of stimulation to evoke firing in highly active and “leader” sites of the network, reliably and rapidly recruited within both spontaneous and evoked bursts. Our study provides the first evidence that spontaneous and evoked activity similarity is reliably observed also in dissociated cortical networks. Nature Publishing Group UK 2017-08-22 /pmc/articles/PMC5567348/ /pubmed/28831071 http://dx.doi.org/10.1038/s41598-017-08369-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pasquale, Valentina
Martinoia, Sergio
Chiappalone, Michela
Stimulation triggers endogenous activity patterns in cultured cortical networks
title Stimulation triggers endogenous activity patterns in cultured cortical networks
title_full Stimulation triggers endogenous activity patterns in cultured cortical networks
title_fullStr Stimulation triggers endogenous activity patterns in cultured cortical networks
title_full_unstemmed Stimulation triggers endogenous activity patterns in cultured cortical networks
title_short Stimulation triggers endogenous activity patterns in cultured cortical networks
title_sort stimulation triggers endogenous activity patterns in cultured cortical networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567348/
https://www.ncbi.nlm.nih.gov/pubmed/28831071
http://dx.doi.org/10.1038/s41598-017-08369-0
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