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Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids

BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based b...

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Autores principales: Zhao, Chen, Fang, Hao, Chen, Shaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568358/
https://www.ncbi.nlm.nih.gov/pubmed/28852423
http://dx.doi.org/10.1186/s13068-017-0889-7
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author Zhao, Chen
Fang, Hao
Chen, Shaolin
author_facet Zhao, Chen
Fang, Hao
Chen, Shaolin
author_sort Zhao, Chen
collection PubMed
description BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86.67 ± 4.06%. Three-stage enzymatic hydrolysis could greatly improve the efficiency of the enzymatic hydrolysis of SECS, achieving a yield of 74.24 ± 2.69% within 30 h. Different bioprocesses from SECS to SCO were compared and the bioprocess C with the three-stage enzymatic hydrolysis was the most efficient, producing 57.15 g dry cell biomass containing 31.80 g SCO from 327.63 g SECS. An efficient and comprehensive process from corn stover to long-chain α,ω-dicarboxylic acids (DCAs) was established by employing self-metathesis, capable of producing 6.02 g long-chain DCAs from 409.54 g corn stover and 6.02 g alkenes as byproducts. CONCLUSIONS: On-site cellulase production by the mixed culture of T. reesei and A. niger is proven the most efficient in providing cellulase to the lignocellulose-based bioprocess. Three-stage enzymatic hydrolysis was found to have very good application value in SCO production by Trichosporon cutaneum from SECS. A whole process from corn stover to long-chain DCAs via a combination of biological and chemical approaches was successfully established and it is an enlightening example of the comprehensive utilization of agricultural wastes.
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spelling pubmed-55683582017-08-29 Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids Zhao, Chen Fang, Hao Chen, Shaolin Biotechnol Biofuels Research BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86.67 ± 4.06%. Three-stage enzymatic hydrolysis could greatly improve the efficiency of the enzymatic hydrolysis of SECS, achieving a yield of 74.24 ± 2.69% within 30 h. Different bioprocesses from SECS to SCO were compared and the bioprocess C with the three-stage enzymatic hydrolysis was the most efficient, producing 57.15 g dry cell biomass containing 31.80 g SCO from 327.63 g SECS. An efficient and comprehensive process from corn stover to long-chain α,ω-dicarboxylic acids (DCAs) was established by employing self-metathesis, capable of producing 6.02 g long-chain DCAs from 409.54 g corn stover and 6.02 g alkenes as byproducts. CONCLUSIONS: On-site cellulase production by the mixed culture of T. reesei and A. niger is proven the most efficient in providing cellulase to the lignocellulose-based bioprocess. Three-stage enzymatic hydrolysis was found to have very good application value in SCO production by Trichosporon cutaneum from SECS. A whole process from corn stover to long-chain DCAs via a combination of biological and chemical approaches was successfully established and it is an enlightening example of the comprehensive utilization of agricultural wastes. BioMed Central 2017-08-23 /pmc/articles/PMC5568358/ /pubmed/28852423 http://dx.doi.org/10.1186/s13068-017-0889-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhao, Chen
Fang, Hao
Chen, Shaolin
Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title_full Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title_fullStr Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title_full_unstemmed Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title_short Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
title_sort single cell oil production by trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568358/
https://www.ncbi.nlm.nih.gov/pubmed/28852423
http://dx.doi.org/10.1186/s13068-017-0889-7
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