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Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids
BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based b...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568358/ https://www.ncbi.nlm.nih.gov/pubmed/28852423 http://dx.doi.org/10.1186/s13068-017-0889-7 |
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author | Zhao, Chen Fang, Hao Chen, Shaolin |
author_facet | Zhao, Chen Fang, Hao Chen, Shaolin |
author_sort | Zhao, Chen |
collection | PubMed |
description | BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86.67 ± 4.06%. Three-stage enzymatic hydrolysis could greatly improve the efficiency of the enzymatic hydrolysis of SECS, achieving a yield of 74.24 ± 2.69% within 30 h. Different bioprocesses from SECS to SCO were compared and the bioprocess C with the three-stage enzymatic hydrolysis was the most efficient, producing 57.15 g dry cell biomass containing 31.80 g SCO from 327.63 g SECS. An efficient and comprehensive process from corn stover to long-chain α,ω-dicarboxylic acids (DCAs) was established by employing self-metathesis, capable of producing 6.02 g long-chain DCAs from 409.54 g corn stover and 6.02 g alkenes as byproducts. CONCLUSIONS: On-site cellulase production by the mixed culture of T. reesei and A. niger is proven the most efficient in providing cellulase to the lignocellulose-based bioprocess. Three-stage enzymatic hydrolysis was found to have very good application value in SCO production by Trichosporon cutaneum from SECS. A whole process from corn stover to long-chain DCAs via a combination of biological and chemical approaches was successfully established and it is an enlightening example of the comprehensive utilization of agricultural wastes. |
format | Online Article Text |
id | pubmed-5568358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55683582017-08-29 Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids Zhao, Chen Fang, Hao Chen, Shaolin Biotechnol Biofuels Research BACKGROUND: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue. RESULTS: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86.67 ± 4.06%. Three-stage enzymatic hydrolysis could greatly improve the efficiency of the enzymatic hydrolysis of SECS, achieving a yield of 74.24 ± 2.69% within 30 h. Different bioprocesses from SECS to SCO were compared and the bioprocess C with the three-stage enzymatic hydrolysis was the most efficient, producing 57.15 g dry cell biomass containing 31.80 g SCO from 327.63 g SECS. An efficient and comprehensive process from corn stover to long-chain α,ω-dicarboxylic acids (DCAs) was established by employing self-metathesis, capable of producing 6.02 g long-chain DCAs from 409.54 g corn stover and 6.02 g alkenes as byproducts. CONCLUSIONS: On-site cellulase production by the mixed culture of T. reesei and A. niger is proven the most efficient in providing cellulase to the lignocellulose-based bioprocess. Three-stage enzymatic hydrolysis was found to have very good application value in SCO production by Trichosporon cutaneum from SECS. A whole process from corn stover to long-chain DCAs via a combination of biological and chemical approaches was successfully established and it is an enlightening example of the comprehensive utilization of agricultural wastes. BioMed Central 2017-08-23 /pmc/articles/PMC5568358/ /pubmed/28852423 http://dx.doi.org/10.1186/s13068-017-0889-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhao, Chen Fang, Hao Chen, Shaolin Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title | Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title_full | Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title_fullStr | Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title_full_unstemmed | Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title_short | Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
title_sort | single cell oil production by trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568358/ https://www.ncbi.nlm.nih.gov/pubmed/28852423 http://dx.doi.org/10.1186/s13068-017-0889-7 |
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