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Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios

We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exem...

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Autores principales: Nazario-Yepiz, Nestor O., Loustalot-Laclette, Mariana Ramirez, Carpinteyro-Ponce, Javier, Abreu-Goodger, Cei, Markow, Therese Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568408/
https://www.ncbi.nlm.nih.gov/pubmed/28832647
http://dx.doi.org/10.1371/journal.pone.0183007
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author Nazario-Yepiz, Nestor O.
Loustalot-Laclette, Mariana Ramirez
Carpinteyro-Ponce, Javier
Abreu-Goodger, Cei
Markow, Therese Ann
author_facet Nazario-Yepiz, Nestor O.
Loustalot-Laclette, Mariana Ramirez
Carpinteyro-Ponce, Javier
Abreu-Goodger, Cei
Markow, Therese Ann
author_sort Nazario-Yepiz, Nestor O.
collection PubMed
description We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exemplifies individuals long exposed to a Western diet higher in sugar, while the natural diet of the cactophilic D. mojavensis, is much lower in carbohydrates. Drosophila arizonae, the sister species of D. mojavensis, is largely cactophilic, but also utilizes rotting fruits that are higher in sugars than cacti. We exposed third instar larvae for 24 hours to diets either (1) high in protein relative to sugar, (2) diets with equal amounts of protein and sugar, and (3) diets low in protein but high in sugar. As we predicted, based upon earlier interspecific studies of development and metabolism, the most extreme differences in gene expression under different dietary conditions were found in D. mojavensis followed by D. arizonae. No differential expression among diets was observed for D. melanogaster, a species that survives well under all three conditions, with little impact on its metabolism. We suggest that these three species together provide a model to examine individual and population differences in vulnerability to lifestyle-associated health problems such as metabolic syndrome and diabetes.
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spelling pubmed-55684082017-09-09 Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios Nazario-Yepiz, Nestor O. Loustalot-Laclette, Mariana Ramirez Carpinteyro-Ponce, Javier Abreu-Goodger, Cei Markow, Therese Ann PLoS One Research Article We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exemplifies individuals long exposed to a Western diet higher in sugar, while the natural diet of the cactophilic D. mojavensis, is much lower in carbohydrates. Drosophila arizonae, the sister species of D. mojavensis, is largely cactophilic, but also utilizes rotting fruits that are higher in sugars than cacti. We exposed third instar larvae for 24 hours to diets either (1) high in protein relative to sugar, (2) diets with equal amounts of protein and sugar, and (3) diets low in protein but high in sugar. As we predicted, based upon earlier interspecific studies of development and metabolism, the most extreme differences in gene expression under different dietary conditions were found in D. mojavensis followed by D. arizonae. No differential expression among diets was observed for D. melanogaster, a species that survives well under all three conditions, with little impact on its metabolism. We suggest that these three species together provide a model to examine individual and population differences in vulnerability to lifestyle-associated health problems such as metabolic syndrome and diabetes. Public Library of Science 2017-08-23 /pmc/articles/PMC5568408/ /pubmed/28832647 http://dx.doi.org/10.1371/journal.pone.0183007 Text en © 2017 Nazario-Yepiz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nazario-Yepiz, Nestor O.
Loustalot-Laclette, Mariana Ramirez
Carpinteyro-Ponce, Javier
Abreu-Goodger, Cei
Markow, Therese Ann
Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title_full Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title_fullStr Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title_full_unstemmed Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title_short Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
title_sort transcriptional responses of ecologically diverse drosophila species to larval diets differing in relative sugar and protein ratios
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568408/
https://www.ncbi.nlm.nih.gov/pubmed/28832647
http://dx.doi.org/10.1371/journal.pone.0183007
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