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Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios
We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exem...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568408/ https://www.ncbi.nlm.nih.gov/pubmed/28832647 http://dx.doi.org/10.1371/journal.pone.0183007 |
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author | Nazario-Yepiz, Nestor O. Loustalot-Laclette, Mariana Ramirez Carpinteyro-Ponce, Javier Abreu-Goodger, Cei Markow, Therese Ann |
author_facet | Nazario-Yepiz, Nestor O. Loustalot-Laclette, Mariana Ramirez Carpinteyro-Ponce, Javier Abreu-Goodger, Cei Markow, Therese Ann |
author_sort | Nazario-Yepiz, Nestor O. |
collection | PubMed |
description | We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exemplifies individuals long exposed to a Western diet higher in sugar, while the natural diet of the cactophilic D. mojavensis, is much lower in carbohydrates. Drosophila arizonae, the sister species of D. mojavensis, is largely cactophilic, but also utilizes rotting fruits that are higher in sugars than cacti. We exposed third instar larvae for 24 hours to diets either (1) high in protein relative to sugar, (2) diets with equal amounts of protein and sugar, and (3) diets low in protein but high in sugar. As we predicted, based upon earlier interspecific studies of development and metabolism, the most extreme differences in gene expression under different dietary conditions were found in D. mojavensis followed by D. arizonae. No differential expression among diets was observed for D. melanogaster, a species that survives well under all three conditions, with little impact on its metabolism. We suggest that these three species together provide a model to examine individual and population differences in vulnerability to lifestyle-associated health problems such as metabolic syndrome and diabetes. |
format | Online Article Text |
id | pubmed-5568408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55684082017-09-09 Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios Nazario-Yepiz, Nestor O. Loustalot-Laclette, Mariana Ramirez Carpinteyro-Ponce, Javier Abreu-Goodger, Cei Markow, Therese Ann PLoS One Research Article We utilized three ecologically diverse Drosophila species to explore the influence of ecological adaptation on transcriptomic responses to isocaloric diets differing in their relative proportions of protein to sugar. Drosophila melanogaster, a cosmopolitan species that breeds in decaying fruit, exemplifies individuals long exposed to a Western diet higher in sugar, while the natural diet of the cactophilic D. mojavensis, is much lower in carbohydrates. Drosophila arizonae, the sister species of D. mojavensis, is largely cactophilic, but also utilizes rotting fruits that are higher in sugars than cacti. We exposed third instar larvae for 24 hours to diets either (1) high in protein relative to sugar, (2) diets with equal amounts of protein and sugar, and (3) diets low in protein but high in sugar. As we predicted, based upon earlier interspecific studies of development and metabolism, the most extreme differences in gene expression under different dietary conditions were found in D. mojavensis followed by D. arizonae. No differential expression among diets was observed for D. melanogaster, a species that survives well under all three conditions, with little impact on its metabolism. We suggest that these three species together provide a model to examine individual and population differences in vulnerability to lifestyle-associated health problems such as metabolic syndrome and diabetes. Public Library of Science 2017-08-23 /pmc/articles/PMC5568408/ /pubmed/28832647 http://dx.doi.org/10.1371/journal.pone.0183007 Text en © 2017 Nazario-Yepiz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nazario-Yepiz, Nestor O. Loustalot-Laclette, Mariana Ramirez Carpinteyro-Ponce, Javier Abreu-Goodger, Cei Markow, Therese Ann Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title | Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title_full | Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title_fullStr | Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title_full_unstemmed | Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title_short | Transcriptional responses of ecologically diverse Drosophila species to larval diets differing in relative sugar and protein ratios |
title_sort | transcriptional responses of ecologically diverse drosophila species to larval diets differing in relative sugar and protein ratios |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568408/ https://www.ncbi.nlm.nih.gov/pubmed/28832647 http://dx.doi.org/10.1371/journal.pone.0183007 |
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