Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia

Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed th...

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Autores principales: Julian, Guilherme Silva, de Oliveira, Renato Watanabe, Tufik, Sergio, Chagas, Jair Ribeiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Pneumologia e Tisiologia 2016
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569605/
https://www.ncbi.nlm.nih.gov/pubmed/27383935
http://dx.doi.org/10.1590/S1806-37562015000000133
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author Julian, Guilherme Silva
de Oliveira, Renato Watanabe
Tufik, Sergio
Chagas, Jair Ribeiro
author_facet Julian, Guilherme Silva
de Oliveira, Renato Watanabe
Tufik, Sergio
Chagas, Jair Ribeiro
author_sort Julian, Guilherme Silva
collection PubMed
description Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA) on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2(−ΔCt) (threshold cycle) data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised.
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spelling pubmed-55696052019-03-19 Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia Julian, Guilherme Silva de Oliveira, Renato Watanabe Tufik, Sergio Chagas, Jair Ribeiro J Bras Pneumol Brief Communication Obstructive sleep apnea (OSA) has been associated with oxidative stress and various cardiovascular consequences, such as increased cardiovascular disease risk. Quantitative real-time PCR is frequently employed to assess changes in gene expression in experimental models. In this study, we analyzed the effects of chronic intermittent hypoxia (an experimental model of OSA) on housekeeping gene expression in the left cardiac ventricle of rats. Analyses via four different approaches-use of the geNorm, BestKeeper, and NormFinder algorithms; and 2(−ΔCt) (threshold cycle) data analysis-produced similar results: all genes were found to be suitable for use, glyceraldehyde-3-phosphate dehydrogenase and 18S being classified as the most and the least stable, respectively. The use of more than one housekeeping gene is strongly advised. Sociedade Brasileira de Pneumologia e Tisiologia 2016 /pmc/articles/PMC5569605/ /pubmed/27383935 http://dx.doi.org/10.1590/S1806-37562015000000133 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Brief Communication
Julian, Guilherme Silva
de Oliveira, Renato Watanabe
Tufik, Sergio
Chagas, Jair Ribeiro
Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title_full Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title_fullStr Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title_full_unstemmed Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title_short Analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
title_sort analysis of the stability of housekeeping gene expression in the left cardiac ventricle of rats submitted to chronic intermittent hypoxia
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569605/
https://www.ncbi.nlm.nih.gov/pubmed/27383935
http://dx.doi.org/10.1590/S1806-37562015000000133
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