Cargando…
A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells
The lacrimal gland secretes tear fluids to ocular surface, which plays an indispensable role in maintaining the health of the ocular epithelia and protecting the ocular surface from the external environment. The dysfunction of the lacrimal glands causes dry eye disease due to a reduction in tear vol...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569877/ https://www.ncbi.nlm.nih.gov/pubmed/28874911 http://dx.doi.org/10.1155/2017/4923426 |
_version_ | 1783259076605509632 |
---|---|
author | Liu, Ying Hirayama, Masatoshi Kawakita, Tetsuya Tsubota, Kazuo |
author_facet | Liu, Ying Hirayama, Masatoshi Kawakita, Tetsuya Tsubota, Kazuo |
author_sort | Liu, Ying |
collection | PubMed |
description | The lacrimal gland secretes tear fluids to ocular surface, which plays an indispensable role in maintaining the health of the ocular epithelia and protecting the ocular surface from the external environment. The dysfunction of the lacrimal glands causes dry eye disease due to a reduction in tear volume. The dry eye disease is becoming a popular public disease, for the number of patients is increasing, who have subjective symptom and loss of vision, which affect the quality of life. Inflammatory change in the damaged lacrimal gland has been reported; however, a major challenge is to establish a simple animal model to observe the changes. Here, we demonstrated an injury model by ligating the main excretory duct of the lacrimal gland, which is a simple and stable way to clearly understand the mechanism of lacrimal gland inflammation. We observed the process of injury and proliferation of the lacrimal gland and detected a population of lacrimal gland epithelial cells with proliferation potential which were also nestin-positive cells following duct ligation. This study successfully established an injury model to observe the tissue injury process of the lacrimal gland, and this model will be useful for analysis of the inflammation and proliferation mechanism in the future. |
format | Online Article Text |
id | pubmed-5569877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-55698772017-09-05 A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells Liu, Ying Hirayama, Masatoshi Kawakita, Tetsuya Tsubota, Kazuo Stem Cells Int Research Article The lacrimal gland secretes tear fluids to ocular surface, which plays an indispensable role in maintaining the health of the ocular epithelia and protecting the ocular surface from the external environment. The dysfunction of the lacrimal glands causes dry eye disease due to a reduction in tear volume. The dry eye disease is becoming a popular public disease, for the number of patients is increasing, who have subjective symptom and loss of vision, which affect the quality of life. Inflammatory change in the damaged lacrimal gland has been reported; however, a major challenge is to establish a simple animal model to observe the changes. Here, we demonstrated an injury model by ligating the main excretory duct of the lacrimal gland, which is a simple and stable way to clearly understand the mechanism of lacrimal gland inflammation. We observed the process of injury and proliferation of the lacrimal gland and detected a population of lacrimal gland epithelial cells with proliferation potential which were also nestin-positive cells following duct ligation. This study successfully established an injury model to observe the tissue injury process of the lacrimal gland, and this model will be useful for analysis of the inflammation and proliferation mechanism in the future. Hindawi 2017 2017-08-10 /pmc/articles/PMC5569877/ /pubmed/28874911 http://dx.doi.org/10.1155/2017/4923426 Text en Copyright © 2017 Ying Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Ying Hirayama, Masatoshi Kawakita, Tetsuya Tsubota, Kazuo A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title | A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title_full | A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title_fullStr | A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title_full_unstemmed | A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title_short | A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells |
title_sort | ligation of the lacrimal excretory duct in mouse induces lacrimal gland inflammation with proliferative cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569877/ https://www.ncbi.nlm.nih.gov/pubmed/28874911 http://dx.doi.org/10.1155/2017/4923426 |
work_keys_str_mv | AT liuying aligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT hirayamamasatoshi aligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT kawakitatetsuya aligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT tsubotakazuo aligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT liuying ligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT hirayamamasatoshi ligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT kawakitatetsuya ligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells AT tsubotakazuo ligationofthelacrimalexcretoryductinmouseinduceslacrimalglandinflammationwithproliferativecells |