Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal...

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Autores principales: Franco-Garcia, Alexandra, Varughese, Tilly A, Lee, Yeon Joo, Papanicolaou, Genovefa, Rosenblum, Marc K, Hollmann, Travis J, Koehne, Guenther, Boulad, Farid, Babady, N Esther, Tang, Yi-Wei, Seo, Susan K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570015/
https://www.ncbi.nlm.nih.gov/pubmed/28852679
http://dx.doi.org/10.1093/ofid/ofx140
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author Franco-Garcia, Alexandra
Varughese, Tilly A
Lee, Yeon Joo
Papanicolaou, Genovefa
Rosenblum, Marc K
Hollmann, Travis J
Koehne, Guenther
Boulad, Farid
Babady, N Esther
Tang, Yi-Wei
Seo, Susan K
author_facet Franco-Garcia, Alexandra
Varughese, Tilly A
Lee, Yeon Joo
Papanicolaou, Genovefa
Rosenblum, Marc K
Hollmann, Travis J
Koehne, Guenther
Boulad, Farid
Babady, N Esther
Tang, Yi-Wei
Seo, Susan K
author_sort Franco-Garcia, Alexandra
collection PubMed
description Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
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spelling pubmed-55700152017-08-29 Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Franco-Garcia, Alexandra Varughese, Tilly A Lee, Yeon Joo Papanicolaou, Genovefa Rosenblum, Marc K Hollmann, Travis J Koehne, Guenther Boulad, Farid Babady, N Esther Tang, Yi-Wei Seo, Susan K Open Forum Infect Dis Brief Report Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxford University Press 2017-07-13 /pmc/articles/PMC5570015/ /pubmed/28852679 http://dx.doi.org/10.1093/ofid/ofx140 Text en © The Author 2017. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Report
Franco-Garcia, Alexandra
Varughese, Tilly A
Lee, Yeon Joo
Papanicolaou, Genovefa
Rosenblum, Marc K
Hollmann, Travis J
Koehne, Guenther
Boulad, Farid
Babady, N Esther
Tang, Yi-Wei
Seo, Susan K
Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_full Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_fullStr Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_full_unstemmed Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_short Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
title_sort diagnosis of extrapulmonary legionellosis in allogeneic hematopoietic cell transplant recipients by direct 16s ribosomal ribonucleic acid sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570015/
https://www.ncbi.nlm.nih.gov/pubmed/28852679
http://dx.doi.org/10.1093/ofid/ofx140
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