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Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells
Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scan...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570135/ https://www.ncbi.nlm.nih.gov/pubmed/28472469 http://dx.doi.org/10.1093/nar/gkx355 |
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author | Ilyin, Artem A. Ryazansky, Sergei S. Doronin, Semen A. Olenkina, Oxana M. Mikhaleva, Elena A. Yakushev, Evgeny Y. Abramov, Yuri A. Belyakin, Stepan N. Ivankin, Anton V. Pindyurin, Alexey V. Gvozdev, Vladimir A. Klenov, Mikhail S. Shevelyov, Yuri Y. |
author_facet | Ilyin, Artem A. Ryazansky, Sergei S. Doronin, Semen A. Olenkina, Oxana M. Mikhaleva, Elena A. Yakushev, Evgeny Y. Abramov, Yuri A. Belyakin, Stepan N. Ivankin, Anton V. Pindyurin, Alexey V. Gvozdev, Vladimir A. Klenov, Mikhail S. Shevelyov, Yuri Y. |
author_sort | Ilyin, Artem A. |
collection | PubMed |
description | Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Here we report the DamID-seq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the protein-coding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts. |
format | Online Article Text |
id | pubmed-5570135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55701352017-08-29 Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells Ilyin, Artem A. Ryazansky, Sergei S. Doronin, Semen A. Olenkina, Oxana M. Mikhaleva, Elena A. Yakushev, Evgeny Y. Abramov, Yuri A. Belyakin, Stepan N. Ivankin, Anton V. Pindyurin, Alexey V. Gvozdev, Vladimir A. Klenov, Mikhail S. Shevelyov, Yuri Y. Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Here we report the DamID-seq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the protein-coding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts. Oxford University Press 2017-07-27 2017-05-02 /pmc/articles/PMC5570135/ /pubmed/28472469 http://dx.doi.org/10.1093/nar/gkx355 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Ilyin, Artem A. Ryazansky, Sergei S. Doronin, Semen A. Olenkina, Oxana M. Mikhaleva, Elena A. Yakushev, Evgeny Y. Abramov, Yuri A. Belyakin, Stepan N. Ivankin, Anton V. Pindyurin, Alexey V. Gvozdev, Vladimir A. Klenov, Mikhail S. Shevelyov, Yuri Y. Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title | Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title_full | Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title_fullStr | Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title_full_unstemmed | Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title_short | Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells |
title_sort | piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in drosophila ovarian somatic cells |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570135/ https://www.ncbi.nlm.nih.gov/pubmed/28472469 http://dx.doi.org/10.1093/nar/gkx355 |
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