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Shared features of cryptic plasmids from environmental and pathogenic Francisella species

The Francisella genus includes several recognized species, additional potential species, and other representatives that inhabit a range of incredibly diverse ecological niches, but are not closely related to the named species. Francisella species have been obtained from a wide variety of clinical an...

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Autores principales: Challacombe, Jean F., Pillai, Segaran, Kuske, Cheryl R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570271/
https://www.ncbi.nlm.nih.gov/pubmed/28837612
http://dx.doi.org/10.1371/journal.pone.0183554
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author Challacombe, Jean F.
Pillai, Segaran
Kuske, Cheryl R.
author_facet Challacombe, Jean F.
Pillai, Segaran
Kuske, Cheryl R.
author_sort Challacombe, Jean F.
collection PubMed
description The Francisella genus includes several recognized species, additional potential species, and other representatives that inhabit a range of incredibly diverse ecological niches, but are not closely related to the named species. Francisella species have been obtained from a wide variety of clinical and environmental sources; documented species include highly virulent human and animal pathogens, fish pathogens, opportunistic human pathogens, tick endosymbionts, and free-living isolates inhabiting brackish water. While more than 120 Francisella genomes have been sequenced to date, only a few contain plasmids, and most of these appear to be cryptic, with unknown benefit to the host cell. We have identified several putative cryptic plasmids in the sequenced genomes of three Francisella novicida and F. novicida-like strains (TX07-6608, AZ06-7470, DPG_3A-IS) and two new Francisella species (F. frigiditurris CA97-1460 and F. opportunistica MA06-7296). These plasmids were compared to each other and to previously identified plasmids from other Francisella species. Some of the plasmids encoded functions potentially involved in replication, conjugal transfer and partitioning, environmental survival (transcriptional regulation, signaling, metabolism), and hypothetical proteins with no assignable functions. Genomic and phylogenetic comparisons of these new plasmids to the other known Francisella plasmids revealed some similarities that add to our understanding of the evolutionary relationships among the diverse Francisella species.
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spelling pubmed-55702712017-09-09 Shared features of cryptic plasmids from environmental and pathogenic Francisella species Challacombe, Jean F. Pillai, Segaran Kuske, Cheryl R. PLoS One Research Article The Francisella genus includes several recognized species, additional potential species, and other representatives that inhabit a range of incredibly diverse ecological niches, but are not closely related to the named species. Francisella species have been obtained from a wide variety of clinical and environmental sources; documented species include highly virulent human and animal pathogens, fish pathogens, opportunistic human pathogens, tick endosymbionts, and free-living isolates inhabiting brackish water. While more than 120 Francisella genomes have been sequenced to date, only a few contain plasmids, and most of these appear to be cryptic, with unknown benefit to the host cell. We have identified several putative cryptic plasmids in the sequenced genomes of three Francisella novicida and F. novicida-like strains (TX07-6608, AZ06-7470, DPG_3A-IS) and two new Francisella species (F. frigiditurris CA97-1460 and F. opportunistica MA06-7296). These plasmids were compared to each other and to previously identified plasmids from other Francisella species. Some of the plasmids encoded functions potentially involved in replication, conjugal transfer and partitioning, environmental survival (transcriptional regulation, signaling, metabolism), and hypothetical proteins with no assignable functions. Genomic and phylogenetic comparisons of these new plasmids to the other known Francisella plasmids revealed some similarities that add to our understanding of the evolutionary relationships among the diverse Francisella species. Public Library of Science 2017-08-24 /pmc/articles/PMC5570271/ /pubmed/28837612 http://dx.doi.org/10.1371/journal.pone.0183554 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Challacombe, Jean F.
Pillai, Segaran
Kuske, Cheryl R.
Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title_full Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title_fullStr Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title_full_unstemmed Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title_short Shared features of cryptic plasmids from environmental and pathogenic Francisella species
title_sort shared features of cryptic plasmids from environmental and pathogenic francisella species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570271/
https://www.ncbi.nlm.nih.gov/pubmed/28837612
http://dx.doi.org/10.1371/journal.pone.0183554
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