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Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development
Zinc deficiency and chronic low level exposures to inorganic arsenic in drinking water are both significant public health concerns that affect millions of people including pregnant women. These two conditions can co-exist in the human population but little is known about their interaction, and in pa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570330/ https://www.ncbi.nlm.nih.gov/pubmed/28837703 http://dx.doi.org/10.1371/journal.pone.0183831 |
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author | Beaver, Laura M. Truong, Lisa Barton, Carrie L. Chase, Tyler T. Gonnerman, Greg D. Wong, Carmen P. Tanguay, Robert L. Ho, Emily |
author_facet | Beaver, Laura M. Truong, Lisa Barton, Carrie L. Chase, Tyler T. Gonnerman, Greg D. Wong, Carmen P. Tanguay, Robert L. Ho, Emily |
author_sort | Beaver, Laura M. |
collection | PubMed |
description | Zinc deficiency and chronic low level exposures to inorganic arsenic in drinking water are both significant public health concerns that affect millions of people including pregnant women. These two conditions can co-exist in the human population but little is known about their interaction, and in particular, whether zinc deficiency sensitizes individuals to arsenic exposure and toxicity, especially during critical windows of development. To address this, we utilized the Danio rerio (zebrafish) model to test the hypothesis that parental zinc deficiency sensitizes the developing embryo to low-concentration arsenic toxicity, leading to altered developmental outcomes. Adult zebrafish were fed defined zinc deficient and zinc adequate diets and were spawned resulting in zinc adequate and zinc deficient embryos. The embryos were treated with environmentally relevant concentrations of 0, 50, and 500 ppb arsenic. Arsenic exposure significantly reduced the amount of zinc in the developing embryo by ~7%. The combination of zinc deficiency and low-level arsenic exposures did not sensitize the developing embryo to increased developmental malformations or mortality. The combination did cause a 40% decline in physical activity of the embryos, and this decline was significantly greater than what was observed with zinc deficiency or arsenic exposure alone. Significant changes in RNA expression of genes that regulate zinc homeostasis, response to oxidative stress and insulin production (including zip1, znt7, nrf2, ogg1, pax4, and insa) were found in zinc deficient, or zinc deficiency and arsenic exposed embryos. Overall, the data suggests that the combination of zinc deficiency and arsenic exposure has harmful effects on the developing embryo and may increase the risk for developing chronic diseases like diabetes. |
format | Online Article Text |
id | pubmed-5570330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55703302017-09-09 Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development Beaver, Laura M. Truong, Lisa Barton, Carrie L. Chase, Tyler T. Gonnerman, Greg D. Wong, Carmen P. Tanguay, Robert L. Ho, Emily PLoS One Research Article Zinc deficiency and chronic low level exposures to inorganic arsenic in drinking water are both significant public health concerns that affect millions of people including pregnant women. These two conditions can co-exist in the human population but little is known about their interaction, and in particular, whether zinc deficiency sensitizes individuals to arsenic exposure and toxicity, especially during critical windows of development. To address this, we utilized the Danio rerio (zebrafish) model to test the hypothesis that parental zinc deficiency sensitizes the developing embryo to low-concentration arsenic toxicity, leading to altered developmental outcomes. Adult zebrafish were fed defined zinc deficient and zinc adequate diets and were spawned resulting in zinc adequate and zinc deficient embryos. The embryos were treated with environmentally relevant concentrations of 0, 50, and 500 ppb arsenic. Arsenic exposure significantly reduced the amount of zinc in the developing embryo by ~7%. The combination of zinc deficiency and low-level arsenic exposures did not sensitize the developing embryo to increased developmental malformations or mortality. The combination did cause a 40% decline in physical activity of the embryos, and this decline was significantly greater than what was observed with zinc deficiency or arsenic exposure alone. Significant changes in RNA expression of genes that regulate zinc homeostasis, response to oxidative stress and insulin production (including zip1, znt7, nrf2, ogg1, pax4, and insa) were found in zinc deficient, or zinc deficiency and arsenic exposed embryos. Overall, the data suggests that the combination of zinc deficiency and arsenic exposure has harmful effects on the developing embryo and may increase the risk for developing chronic diseases like diabetes. Public Library of Science 2017-08-24 /pmc/articles/PMC5570330/ /pubmed/28837703 http://dx.doi.org/10.1371/journal.pone.0183831 Text en © 2017 Beaver et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Beaver, Laura M. Truong, Lisa Barton, Carrie L. Chase, Tyler T. Gonnerman, Greg D. Wong, Carmen P. Tanguay, Robert L. Ho, Emily Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title | Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title_full | Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title_fullStr | Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title_full_unstemmed | Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title_short | Combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (Danio rerio) development |
title_sort | combinatorial effects of zinc deficiency and arsenic exposure on zebrafish (danio rerio) development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570330/ https://www.ncbi.nlm.nih.gov/pubmed/28837703 http://dx.doi.org/10.1371/journal.pone.0183831 |
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