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Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development

OBJECTIVE: Due to recent progress in production of human embryonic stem cell-derived oligodendrocyte progenitor cells (hESC-OPCs) for ameliorating myelin disease such as multiple sclerosis (MS) and the role of purinergic signaling in OPCs development, we avaluated the profile of purinergic receptors...

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Autores principales: Kashfi, Shirin, Peymani, Maryam, Ghaedi, Kamran, Baharvand, Hossein, Nasr Esfahani, Mohammad Hossein, Javan, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570404/
https://www.ncbi.nlm.nih.gov/pubmed/28836401
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author Kashfi, Shirin
Peymani, Maryam
Ghaedi, Kamran
Baharvand, Hossein
Nasr Esfahani, Mohammad Hossein
Javan, Mohammad
author_facet Kashfi, Shirin
Peymani, Maryam
Ghaedi, Kamran
Baharvand, Hossein
Nasr Esfahani, Mohammad Hossein
Javan, Mohammad
author_sort Kashfi, Shirin
collection PubMed
description OBJECTIVE: Due to recent progress in production of human embryonic stem cell-derived oligodendrocyte progenitor cells (hESC-OPCs) for ameliorating myelin disease such as multiple sclerosis (MS) and the role of purinergic signaling in OPCs development, we avaluated the profile of purinergic receptors expression during development of OPCs from hESC. MATERIALS AND METHODS: In this experimental study, we used reverse transcription and quantitative polymerase chain reaction (RT-qPCR) to obtain more information about potential roles of purinergic receptors during in vitro production of hESC-OPCs. We first determined the expression level of different subtypes of purinergic receptors in hESCs, embryoid bodies (EBs), and hESC-OPCs. The effects of A1adenosine receptor (A(1)AR) activation on hESC-OPCs development were subsequently examined. RESULTS: hESCs and OPCs had different mRNA expression levels of the AR subtypes. ARs mRNA were expressed in the EB stage, except for A(2A)AR. We observed expressions of several P2X (P2X(1, 2, 3, 4, 5, 7)) and P2Y (P2Y(1, 2, 4, 6, 11-14)) genes in hESCs. hESC-OPCs expressed different subtypes of P2X (P2X(1, 2, 3,4,5,7)) and P2Y (P2Y(1, 2, 4, 6, 11-14)). Except for P2X(1) and P2X(6), all other P2X and P2Y purinergic receptor subtypes expressed in EBs. We also indicate that A(1)AR might be involved in modulating gene expression levels of cell cycle regulators in an agonist and/or dose-dependent manner. CONCLUSION: Elucidation of the expression pattern of purinergic receptors and the effects of different subtypes of these receptors in hESC-OPCs may have a promising role in future cell-based therapy or drug design for demyelinating disease.
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spelling pubmed-55704042017-09-01 Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development Kashfi, Shirin Peymani, Maryam Ghaedi, Kamran Baharvand, Hossein Nasr Esfahani, Mohammad Hossein Javan, Mohammad Cell J Original Article OBJECTIVE: Due to recent progress in production of human embryonic stem cell-derived oligodendrocyte progenitor cells (hESC-OPCs) for ameliorating myelin disease such as multiple sclerosis (MS) and the role of purinergic signaling in OPCs development, we avaluated the profile of purinergic receptors expression during development of OPCs from hESC. MATERIALS AND METHODS: In this experimental study, we used reverse transcription and quantitative polymerase chain reaction (RT-qPCR) to obtain more information about potential roles of purinergic receptors during in vitro production of hESC-OPCs. We first determined the expression level of different subtypes of purinergic receptors in hESCs, embryoid bodies (EBs), and hESC-OPCs. The effects of A1adenosine receptor (A(1)AR) activation on hESC-OPCs development were subsequently examined. RESULTS: hESCs and OPCs had different mRNA expression levels of the AR subtypes. ARs mRNA were expressed in the EB stage, except for A(2A)AR. We observed expressions of several P2X (P2X(1, 2, 3, 4, 5, 7)) and P2Y (P2Y(1, 2, 4, 6, 11-14)) genes in hESCs. hESC-OPCs expressed different subtypes of P2X (P2X(1, 2, 3,4,5,7)) and P2Y (P2Y(1, 2, 4, 6, 11-14)). Except for P2X(1) and P2X(6), all other P2X and P2Y purinergic receptor subtypes expressed in EBs. We also indicate that A(1)AR might be involved in modulating gene expression levels of cell cycle regulators in an agonist and/or dose-dependent manner. CONCLUSION: Elucidation of the expression pattern of purinergic receptors and the effects of different subtypes of these receptors in hESC-OPCs may have a promising role in future cell-based therapy or drug design for demyelinating disease. Royan Institute 2017 2017-08-19 /pmc/articles/PMC5570404/ /pubmed/28836401 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kashfi, Shirin
Peymani, Maryam
Ghaedi, Kamran
Baharvand, Hossein
Nasr Esfahani, Mohammad Hossein
Javan, Mohammad
Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title_full Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title_fullStr Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title_full_unstemmed Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title_short Purinergic Receptor Expression and Potential Association with Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Development
title_sort purinergic receptor expression and potential association with human embryonic stem cell-derived oligodendrocyte progenitor cell development
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570404/
https://www.ncbi.nlm.nih.gov/pubmed/28836401
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