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The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence
Shigella spp. causing bacterial diarrhea and dysentery are human enteroinvasive bacterial pathogens that are orally transmitted through contaminated food and water and cause bacillary dysentery. Although natural Shigella infections are restricted to humans and primates, several smaller animal models...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570432/ https://www.ncbi.nlm.nih.gov/pubmed/28277944 http://dx.doi.org/10.1080/19490976.2017.1293225 |
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author | Barnoy, Shoshana Gancz, Hanan Zhu, Yuewei Honnold, Cary L. Zurawski, Daniel V. Venkatesan, Malabi M. |
author_facet | Barnoy, Shoshana Gancz, Hanan Zhu, Yuewei Honnold, Cary L. Zurawski, Daniel V. Venkatesan, Malabi M. |
author_sort | Barnoy, Shoshana |
collection | PubMed |
description | Shigella spp. causing bacterial diarrhea and dysentery are human enteroinvasive bacterial pathogens that are orally transmitted through contaminated food and water and cause bacillary dysentery. Although natural Shigella infections are restricted to humans and primates, several smaller animal models are used to analyze individual steps in pathogenesis. No animal model fully duplicates the human response and sustaining the models requires expensive animals, costly maintenance of animal facilities, veterinary services and approved animal protocols. This study proposes the development of the caterpillar larvae of Galleria mellonella as a simple, inexpensive, informative, and rapid in-vivo model for evaluating virulence and the interaction of Shigella with cells of the insect innate immunity. Virulent Shigella injected through the forelegs causes larvae death. The mortality rates were dependent on the Shigella strain, the infectious dose, and the presence of the virulence plasmid. Wild-type S. flexneri 2a, persisted and replicated within the larvae, resulting in haemocyte cell death, whereas plasmid-cured mutants were rapidly cleared. Histology of the infected larvae in conjunction with fluorescence, immunofluorescence, and transmission electron microscopy indicate that S. flexneri reside within a vacuole of the insect haemocytes that ultrastructurally resembles vacuoles described in studies with mouse and human macrophage cell lines. Some of these bacteria-laden vacuoles had double-membranes characteristic of autophagosomes. These results suggest that G. mellonella larvae can be used as an easy-to-use animal model to understand Shigella pathogenesis that requires none of the time and labor-consuming procedures typical of other systems. |
format | Online Article Text |
id | pubmed-5570432 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-55704322017-08-29 The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence Barnoy, Shoshana Gancz, Hanan Zhu, Yuewei Honnold, Cary L. Zurawski, Daniel V. Venkatesan, Malabi M. Gut Microbes Research Paper/Report Shigella spp. causing bacterial diarrhea and dysentery are human enteroinvasive bacterial pathogens that are orally transmitted through contaminated food and water and cause bacillary dysentery. Although natural Shigella infections are restricted to humans and primates, several smaller animal models are used to analyze individual steps in pathogenesis. No animal model fully duplicates the human response and sustaining the models requires expensive animals, costly maintenance of animal facilities, veterinary services and approved animal protocols. This study proposes the development of the caterpillar larvae of Galleria mellonella as a simple, inexpensive, informative, and rapid in-vivo model for evaluating virulence and the interaction of Shigella with cells of the insect innate immunity. Virulent Shigella injected through the forelegs causes larvae death. The mortality rates were dependent on the Shigella strain, the infectious dose, and the presence of the virulence plasmid. Wild-type S. flexneri 2a, persisted and replicated within the larvae, resulting in haemocyte cell death, whereas plasmid-cured mutants were rapidly cleared. Histology of the infected larvae in conjunction with fluorescence, immunofluorescence, and transmission electron microscopy indicate that S. flexneri reside within a vacuole of the insect haemocytes that ultrastructurally resembles vacuoles described in studies with mouse and human macrophage cell lines. Some of these bacteria-laden vacuoles had double-membranes characteristic of autophagosomes. These results suggest that G. mellonella larvae can be used as an easy-to-use animal model to understand Shigella pathogenesis that requires none of the time and labor-consuming procedures typical of other systems. Taylor & Francis 2017-02-13 /pmc/articles/PMC5570432/ /pubmed/28277944 http://dx.doi.org/10.1080/19490976.2017.1293225 Text en © 2017 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
spellingShingle | Research Paper/Report Barnoy, Shoshana Gancz, Hanan Zhu, Yuewei Honnold, Cary L. Zurawski, Daniel V. Venkatesan, Malabi M. The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title | The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title_full | The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title_fullStr | The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title_full_unstemmed | The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title_short | The Galleria mellonella larvae as an in vivo model for evaluation of Shigella virulence |
title_sort | galleria mellonella larvae as an in vivo model for evaluation of shigella virulence |
topic | Research Paper/Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570432/ https://www.ncbi.nlm.nih.gov/pubmed/28277944 http://dx.doi.org/10.1080/19490976.2017.1293225 |
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