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Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions

The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of (31)P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous...

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Detalles Bibliográficos
Autores principales: Nardi-Schreiber, Atara, Gamliel, Ayelet, Harris, Talia, Sapir, Gal, Sosna, Jacob, Gomori, J. Moshe, Katz-Brull, Rachel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570947/
https://www.ncbi.nlm.nih.gov/pubmed/28839124
http://dx.doi.org/10.1038/s41467-017-00364-3
Descripción
Sumario:The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of (31)P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D(2)O (T(1) 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the (31)P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates.