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Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions

The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of (31)P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous...

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Autores principales: Nardi-Schreiber, Atara, Gamliel, Ayelet, Harris, Talia, Sapir, Gal, Sosna, Jacob, Gomori, J. Moshe, Katz-Brull, Rachel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570947/
https://www.ncbi.nlm.nih.gov/pubmed/28839124
http://dx.doi.org/10.1038/s41467-017-00364-3
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author Nardi-Schreiber, Atara
Gamliel, Ayelet
Harris, Talia
Sapir, Gal
Sosna, Jacob
Gomori, J. Moshe
Katz-Brull, Rachel
author_facet Nardi-Schreiber, Atara
Gamliel, Ayelet
Harris, Talia
Sapir, Gal
Sosna, Jacob
Gomori, J. Moshe
Katz-Brull, Rachel
author_sort Nardi-Schreiber, Atara
collection PubMed
description The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of (31)P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D(2)O (T(1) 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the (31)P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates.
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spelling pubmed-55709472017-08-30 Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions Nardi-Schreiber, Atara Gamliel, Ayelet Harris, Talia Sapir, Gal Sosna, Jacob Gomori, J. Moshe Katz-Brull, Rachel Nat Commun Article The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of (31)P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D(2)O (T(1) 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the (31)P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates. Nature Publishing Group UK 2017-08-24 /pmc/articles/PMC5570947/ /pubmed/28839124 http://dx.doi.org/10.1038/s41467-017-00364-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nardi-Schreiber, Atara
Gamliel, Ayelet
Harris, Talia
Sapir, Gal
Sosna, Jacob
Gomori, J. Moshe
Katz-Brull, Rachel
Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title_full Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title_fullStr Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title_full_unstemmed Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title_short Biochemical phosphates observed using hyperpolarized (31)P in physiological aqueous solutions
title_sort biochemical phosphates observed using hyperpolarized (31)p in physiological aqueous solutions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570947/
https://www.ncbi.nlm.nih.gov/pubmed/28839124
http://dx.doi.org/10.1038/s41467-017-00364-3
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