A Systematic Protein Refolding Screen Method using the DGR Approach Reveals that Time and Secondary TSA are Essential Variables

Refolding of proteins derived from inclusion bodies is very promising as it can provide a reliable source of target proteins of high purity. However, inclusion body-based protein production is often limited by the lack of techniques for the detection of correctly refolded protein. Thus, the selectio...

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Detalles Bibliográficos
Autores principales: Wang, Yuanze, van Oosterwijk, Niels, Ali, Ameena M., Adawy, Alaa, Anindya, Atsarina L., Dömling, Alexander S. S., Groves, Matthew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570958/
https://www.ncbi.nlm.nih.gov/pubmed/28839267
http://dx.doi.org/10.1038/s41598-017-09687-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570958/
https://www.ncbi.nlm.nih.gov/pubmed/28839267
http://dx.doi.org/10.1038/s41598-017-09687-z

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