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A Systematic Protein Refolding Screen Method using the DGR Approach Reveals that Time and Secondary TSA are Essential Variables
Refolding of proteins derived from inclusion bodies is very promising as it can provide a reliable source of target proteins of high purity. However, inclusion body-based protein production is often limited by the lack of techniques for the detection of correctly refolded protein. Thus, the selectio...
Autores principales: | Wang, Yuanze, van Oosterwijk, Niels, Ali, Ameena M., Adawy, Alaa, Anindya, Atsarina L., Dömling, Alexander S. S., Groves, Matthew R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5570958/ https://www.ncbi.nlm.nih.gov/pubmed/28839267 http://dx.doi.org/10.1038/s41598-017-09687-z |
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