Cargando…

The impact of immigration on microbial community composition in full-scale anaerobic digesters

Anaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. Hence, we investigated microbial communities of thirty-two full-scale anaerobi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kirkegaard, Rasmus H., McIlroy, Simon J., Kristensen, Jannie M., Nierychlo, Marta, Karst, Søren M., Dueholm, Morten S., Albertsen, Mads, Nielsen, Per H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571154/
https://www.ncbi.nlm.nih.gov/pubmed/28839166
http://dx.doi.org/10.1038/s41598-017-09303-0
_version_ 1783259300915838976
author Kirkegaard, Rasmus H.
McIlroy, Simon J.
Kristensen, Jannie M.
Nierychlo, Marta
Karst, Søren M.
Dueholm, Morten S.
Albertsen, Mads
Nielsen, Per H.
author_facet Kirkegaard, Rasmus H.
McIlroy, Simon J.
Kristensen, Jannie M.
Nierychlo, Marta
Karst, Søren M.
Dueholm, Morten S.
Albertsen, Mads
Nielsen, Per H.
author_sort Kirkegaard, Rasmus H.
collection PubMed
description Anaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. Hence, we investigated microbial communities of thirty-two full-scale anaerobic digesters over a six-year period using 16S rRNA gene amplicon sequencing. Sampling of the sludge fed into these systems revealed that several of the most abundant populations were likely inactive and immigrating with the influent. This observation indicates that a failure to consider immigration will interfere with correlation analysis and give an inaccurate picture of the growing microbial community. Furthermore, several abundant OTUs could not be classified to genus level with commonly applied taxonomies, making inference of their function unreliable and comparison to other studies problematic. As such, the existing MiDAS taxonomy was updated to include these abundant phylotypes. The communities of individual digesters surveyed were remarkably similar – with only 300 OTUs representing 80% of the total reads across all plants, and 15% of these identified as non-growing and possibly inactive immigrating microbes. By identifying abundant and growing taxa in anaerobic digestion, this study paves the way for targeted characterisation of the process-important organisms towards an in-depth understanding of the microbiology.
format Online
Article
Text
id pubmed-5571154
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55711542017-09-01 The impact of immigration on microbial community composition in full-scale anaerobic digesters Kirkegaard, Rasmus H. McIlroy, Simon J. Kristensen, Jannie M. Nierychlo, Marta Karst, Søren M. Dueholm, Morten S. Albertsen, Mads Nielsen, Per H. Sci Rep Article Anaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. Hence, we investigated microbial communities of thirty-two full-scale anaerobic digesters over a six-year period using 16S rRNA gene amplicon sequencing. Sampling of the sludge fed into these systems revealed that several of the most abundant populations were likely inactive and immigrating with the influent. This observation indicates that a failure to consider immigration will interfere with correlation analysis and give an inaccurate picture of the growing microbial community. Furthermore, several abundant OTUs could not be classified to genus level with commonly applied taxonomies, making inference of their function unreliable and comparison to other studies problematic. As such, the existing MiDAS taxonomy was updated to include these abundant phylotypes. The communities of individual digesters surveyed were remarkably similar – with only 300 OTUs representing 80% of the total reads across all plants, and 15% of these identified as non-growing and possibly inactive immigrating microbes. By identifying abundant and growing taxa in anaerobic digestion, this study paves the way for targeted characterisation of the process-important organisms towards an in-depth understanding of the microbiology. Nature Publishing Group UK 2017-08-24 /pmc/articles/PMC5571154/ /pubmed/28839166 http://dx.doi.org/10.1038/s41598-017-09303-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kirkegaard, Rasmus H.
McIlroy, Simon J.
Kristensen, Jannie M.
Nierychlo, Marta
Karst, Søren M.
Dueholm, Morten S.
Albertsen, Mads
Nielsen, Per H.
The impact of immigration on microbial community composition in full-scale anaerobic digesters
title The impact of immigration on microbial community composition in full-scale anaerobic digesters
title_full The impact of immigration on microbial community composition in full-scale anaerobic digesters
title_fullStr The impact of immigration on microbial community composition in full-scale anaerobic digesters
title_full_unstemmed The impact of immigration on microbial community composition in full-scale anaerobic digesters
title_short The impact of immigration on microbial community composition in full-scale anaerobic digesters
title_sort impact of immigration on microbial community composition in full-scale anaerobic digesters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571154/
https://www.ncbi.nlm.nih.gov/pubmed/28839166
http://dx.doi.org/10.1038/s41598-017-09303-0
work_keys_str_mv AT kirkegaardrasmush theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT mcilroysimonj theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT kristensenjanniem theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT nierychlomarta theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT karstsørenm theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT dueholmmortens theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT albertsenmads theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT nielsenperh theimpactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT kirkegaardrasmush impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT mcilroysimonj impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT kristensenjanniem impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT nierychlomarta impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT karstsørenm impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT dueholmmortens impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT albertsenmads impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters
AT nielsenperh impactofimmigrationonmicrobialcommunitycompositioninfullscaleanaerobicdigesters