Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells

Endothelial cells (EC) are involved in regulating several aspects of lipid metabolism, with recent research revealing the clinicopathological significance of interactions between EC and lipids. Induced pluripotent stem cells (iPSC) have various possible medical uses, so understanding the metabolism...

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Autores principales: Nakamura, Yusuke, Shimizu, Yasuo, Horibata, Yasuhiro, Tei, Rinna, Koike, Ryosuke, Masawa, Meitetsu, Watanabe, Taiji, Shiobara, Taichi, Arai, Ryo, Chibana, Kazuyuki, Takemasa, Akihiro, Sugimoto, Hiroyuki, Ishii, Yoshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571164/
https://www.ncbi.nlm.nih.gov/pubmed/28839272
http://dx.doi.org/10.1038/s41598-017-09980-x
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author Nakamura, Yusuke
Shimizu, Yasuo
Horibata, Yasuhiro
Tei, Rinna
Koike, Ryosuke
Masawa, Meitetsu
Watanabe, Taiji
Shiobara, Taichi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Sugimoto, Hiroyuki
Ishii, Yoshiki
author_facet Nakamura, Yusuke
Shimizu, Yasuo
Horibata, Yasuhiro
Tei, Rinna
Koike, Ryosuke
Masawa, Meitetsu
Watanabe, Taiji
Shiobara, Taichi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Sugimoto, Hiroyuki
Ishii, Yoshiki
author_sort Nakamura, Yusuke
collection PubMed
description Endothelial cells (EC) are involved in regulating several aspects of lipid metabolism, with recent research revealing the clinicopathological significance of interactions between EC and lipids. Induced pluripotent stem cells (iPSC) have various possible medical uses, so understanding the metabolism of these cells is important. In this study, endothelial phenotype cells generated from human iPSC formed cell networks in co-culture with fibroblasts. Changes of plasmalogen lipids and sphingomyelins in endothelial phenotype cells generated from human iPSC were investigated by reverse-phase ultra-high-pressure liquid chromatography mass spectrometry (UHPLC-MS/MS) analysis. The levels of plasmalogen phosphatidylethanolamines (38:5) and (38:4) increased during differentiation of EC, while sphingomyelin levels decreased transiently. These changes of plasmalogen lipids and sphingomyelins may have physiological significance for EC and could be used as markers of differentiation.
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spelling pubmed-55711642017-09-01 Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells Nakamura, Yusuke Shimizu, Yasuo Horibata, Yasuhiro Tei, Rinna Koike, Ryosuke Masawa, Meitetsu Watanabe, Taiji Shiobara, Taichi Arai, Ryo Chibana, Kazuyuki Takemasa, Akihiro Sugimoto, Hiroyuki Ishii, Yoshiki Sci Rep Article Endothelial cells (EC) are involved in regulating several aspects of lipid metabolism, with recent research revealing the clinicopathological significance of interactions between EC and lipids. Induced pluripotent stem cells (iPSC) have various possible medical uses, so understanding the metabolism of these cells is important. In this study, endothelial phenotype cells generated from human iPSC formed cell networks in co-culture with fibroblasts. Changes of plasmalogen lipids and sphingomyelins in endothelial phenotype cells generated from human iPSC were investigated by reverse-phase ultra-high-pressure liquid chromatography mass spectrometry (UHPLC-MS/MS) analysis. The levels of plasmalogen phosphatidylethanolamines (38:5) and (38:4) increased during differentiation of EC, while sphingomyelin levels decreased transiently. These changes of plasmalogen lipids and sphingomyelins may have physiological significance for EC and could be used as markers of differentiation. Nature Publishing Group UK 2017-08-24 /pmc/articles/PMC5571164/ /pubmed/28839272 http://dx.doi.org/10.1038/s41598-017-09980-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nakamura, Yusuke
Shimizu, Yasuo
Horibata, Yasuhiro
Tei, Rinna
Koike, Ryosuke
Masawa, Meitetsu
Watanabe, Taiji
Shiobara, Taichi
Arai, Ryo
Chibana, Kazuyuki
Takemasa, Akihiro
Sugimoto, Hiroyuki
Ishii, Yoshiki
Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title_full Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title_fullStr Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title_full_unstemmed Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title_short Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells
title_sort changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409b2 to endothelial phenotype cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571164/
https://www.ncbi.nlm.nih.gov/pubmed/28839272
http://dx.doi.org/10.1038/s41598-017-09980-x
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