Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques

Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution real-time 3-D refractive index (RI) measurements and dynamic 2-D phase images of individual melittin-bound RBCs...

Descripción completa

Detalles Bibliográficos
Autores principales: Hur, Joonseok, Kim, Kyoohyun, Lee, SangYun, Park, HyunJoo, Park, YongKeun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571175/
https://www.ncbi.nlm.nih.gov/pubmed/28839153
http://dx.doi.org/10.1038/s41598-017-08675-7
_version_ 1783259305789620224
author Hur, Joonseok
Kim, Kyoohyun
Lee, SangYun
Park, HyunJoo
Park, YongKeun
author_facet Hur, Joonseok
Kim, Kyoohyun
Lee, SangYun
Park, HyunJoo
Park, YongKeun
author_sort Hur, Joonseok
collection PubMed
description Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution real-time 3-D refractive index (RI) measurements and dynamic 2-D phase images of individual melittin-bound RBCs enabled in-depth examination of melittin-induced biophysical alterations of the cells. From the measurements, morphological, intracellular, and mechanical alterations of the RBCs were analyzed quantitatively. Furthermore, leakage of haemoglobin (Hb) inside the RBCs at high melittin concentration was also investigated.
format Online
Article
Text
id pubmed-5571175
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55711752017-09-01 Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques Hur, Joonseok Kim, Kyoohyun Lee, SangYun Park, HyunJoo Park, YongKeun Sci Rep Article Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution real-time 3-D refractive index (RI) measurements and dynamic 2-D phase images of individual melittin-bound RBCs enabled in-depth examination of melittin-induced biophysical alterations of the cells. From the measurements, morphological, intracellular, and mechanical alterations of the RBCs were analyzed quantitatively. Furthermore, leakage of haemoglobin (Hb) inside the RBCs at high melittin concentration was also investigated. Nature Publishing Group UK 2017-08-24 /pmc/articles/PMC5571175/ /pubmed/28839153 http://dx.doi.org/10.1038/s41598-017-08675-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hur, Joonseok
Kim, Kyoohyun
Lee, SangYun
Park, HyunJoo
Park, YongKeun
Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title_full Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title_fullStr Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title_full_unstemmed Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title_short Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
title_sort melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571175/
https://www.ncbi.nlm.nih.gov/pubmed/28839153
http://dx.doi.org/10.1038/s41598-017-08675-7
work_keys_str_mv AT hurjoonseok melittininducedalterationsinmorphologyanddeformabilityofhumanredbloodcellsusingquantitativephaseimagingtechniques
AT kimkyoohyun melittininducedalterationsinmorphologyanddeformabilityofhumanredbloodcellsusingquantitativephaseimagingtechniques
AT leesangyun melittininducedalterationsinmorphologyanddeformabilityofhumanredbloodcellsusingquantitativephaseimagingtechniques
AT parkhyunjoo melittininducedalterationsinmorphologyanddeformabilityofhumanredbloodcellsusingquantitativephaseimagingtechniques
AT parkyongkeun melittininducedalterationsinmorphologyanddeformabilityofhumanredbloodcellsusingquantitativephaseimagingtechniques

Ejemplares similares