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Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium

Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on a...

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Autores principales: Wang, Qiang‐li, Wang, Hai‐jie, Li, Zhi‐hua, Wang, Yong‐li, Wu, Xue‐ping, Tan, Yu‐zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571540/
https://www.ncbi.nlm.nih.gov/pubmed/28244640
http://dx.doi.org/10.1111/jcmm.13097
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author Wang, Qiang‐li
Wang, Hai‐jie
Li, Zhi‐hua
Wang, Yong‐li
Wu, Xue‐ping
Tan, Yu‐zhen
author_facet Wang, Qiang‐li
Wang, Hai‐jie
Li, Zhi‐hua
Wang, Yong‐li
Wu, Xue‐ping
Tan, Yu‐zhen
author_sort Wang, Qiang‐li
collection PubMed
description Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF‐1α, Tβ4, VEGF and SDF‐1 expression and decreased CXCL14 expression in hypoxic and serum‐deprived conditions. In murine MI models, the survival and distribution of the engrafted MSCs and the activation of the epicardium were examined, respectively. At 4 weeks after transplantation of the cell patch, the cardiac functions were significantly improved. The engrafted MSCs migrated across the epicardium and into the myocardium. Tendency of HIF‐1α, Tβ4, VEGF, SDF‐1 and CXCL14 expression in the infarcted myocardium was similar with expression in vitro. The epicardium was activated and epicardial‐derived cells (EPDCs) migrated into deep tissue. The EPDCs differentiated into endothelial cells and smooth muscle cells, and some of EPDCs showed to have differentiated into cardiomyocytes. Density of blood and lymphatic capillaries increased significantly. More c‐kit(+) cells were recruited into the infarcted myocardium after transplantation of the cell patch. The results suggest that epicardial transplantation of the cell patch promotes repair of the infarcted myocardium and improves cardiac functions by enhancing the survival of the transplanted cells, accelerating locality paracrine, and then activating the epicardium and recruiting endogenous c‐kit(+) cells. Epicardial transplantation of the cell patch may be applied as a novel effective MI therapy.
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spelling pubmed-55715402017-09-01 Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium Wang, Qiang‐li Wang, Hai‐jie Li, Zhi‐hua Wang, Yong‐li Wu, Xue‐ping Tan, Yu‐zhen J Cell Mol Med Original Articles Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF‐1α, Tβ4, VEGF and SDF‐1 expression and decreased CXCL14 expression in hypoxic and serum‐deprived conditions. In murine MI models, the survival and distribution of the engrafted MSCs and the activation of the epicardium were examined, respectively. At 4 weeks after transplantation of the cell patch, the cardiac functions were significantly improved. The engrafted MSCs migrated across the epicardium and into the myocardium. Tendency of HIF‐1α, Tβ4, VEGF, SDF‐1 and CXCL14 expression in the infarcted myocardium was similar with expression in vitro. The epicardium was activated and epicardial‐derived cells (EPDCs) migrated into deep tissue. The EPDCs differentiated into endothelial cells and smooth muscle cells, and some of EPDCs showed to have differentiated into cardiomyocytes. Density of blood and lymphatic capillaries increased significantly. More c‐kit(+) cells were recruited into the infarcted myocardium after transplantation of the cell patch. The results suggest that epicardial transplantation of the cell patch promotes repair of the infarcted myocardium and improves cardiac functions by enhancing the survival of the transplanted cells, accelerating locality paracrine, and then activating the epicardium and recruiting endogenous c‐kit(+) cells. Epicardial transplantation of the cell patch may be applied as a novel effective MI therapy. John Wiley and Sons Inc. 2017-02-28 2017-09 /pmc/articles/PMC5571540/ /pubmed/28244640 http://dx.doi.org/10.1111/jcmm.13097 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Qiang‐li
Wang, Hai‐jie
Li, Zhi‐hua
Wang, Yong‐li
Wu, Xue‐ping
Tan, Yu‐zhen
Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title_full Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title_fullStr Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title_full_unstemmed Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title_short Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
title_sort mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571540/
https://www.ncbi.nlm.nih.gov/pubmed/28244640
http://dx.doi.org/10.1111/jcmm.13097
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