Cargando…
Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium
Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571540/ https://www.ncbi.nlm.nih.gov/pubmed/28244640 http://dx.doi.org/10.1111/jcmm.13097 |
_version_ | 1783259361252999168 |
---|---|
author | Wang, Qiang‐li Wang, Hai‐jie Li, Zhi‐hua Wang, Yong‐li Wu, Xue‐ping Tan, Yu‐zhen |
author_facet | Wang, Qiang‐li Wang, Hai‐jie Li, Zhi‐hua Wang, Yong‐li Wu, Xue‐ping Tan, Yu‐zhen |
author_sort | Wang, Qiang‐li |
collection | PubMed |
description | Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF‐1α, Tβ4, VEGF and SDF‐1 expression and decreased CXCL14 expression in hypoxic and serum‐deprived conditions. In murine MI models, the survival and distribution of the engrafted MSCs and the activation of the epicardium were examined, respectively. At 4 weeks after transplantation of the cell patch, the cardiac functions were significantly improved. The engrafted MSCs migrated across the epicardium and into the myocardium. Tendency of HIF‐1α, Tβ4, VEGF, SDF‐1 and CXCL14 expression in the infarcted myocardium was similar with expression in vitro. The epicardium was activated and epicardial‐derived cells (EPDCs) migrated into deep tissue. The EPDCs differentiated into endothelial cells and smooth muscle cells, and some of EPDCs showed to have differentiated into cardiomyocytes. Density of blood and lymphatic capillaries increased significantly. More c‐kit(+) cells were recruited into the infarcted myocardium after transplantation of the cell patch. The results suggest that epicardial transplantation of the cell patch promotes repair of the infarcted myocardium and improves cardiac functions by enhancing the survival of the transplanted cells, accelerating locality paracrine, and then activating the epicardium and recruiting endogenous c‐kit(+) cells. Epicardial transplantation of the cell patch may be applied as a novel effective MI therapy. |
format | Online Article Text |
id | pubmed-5571540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55715402017-09-01 Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium Wang, Qiang‐li Wang, Hai‐jie Li, Zhi‐hua Wang, Yong‐li Wu, Xue‐ping Tan, Yu‐zhen J Cell Mol Med Original Articles Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF‐1α, Tβ4, VEGF and SDF‐1 expression and decreased CXCL14 expression in hypoxic and serum‐deprived conditions. In murine MI models, the survival and distribution of the engrafted MSCs and the activation of the epicardium were examined, respectively. At 4 weeks after transplantation of the cell patch, the cardiac functions were significantly improved. The engrafted MSCs migrated across the epicardium and into the myocardium. Tendency of HIF‐1α, Tβ4, VEGF, SDF‐1 and CXCL14 expression in the infarcted myocardium was similar with expression in vitro. The epicardium was activated and epicardial‐derived cells (EPDCs) migrated into deep tissue. The EPDCs differentiated into endothelial cells and smooth muscle cells, and some of EPDCs showed to have differentiated into cardiomyocytes. Density of blood and lymphatic capillaries increased significantly. More c‐kit(+) cells were recruited into the infarcted myocardium after transplantation of the cell patch. The results suggest that epicardial transplantation of the cell patch promotes repair of the infarcted myocardium and improves cardiac functions by enhancing the survival of the transplanted cells, accelerating locality paracrine, and then activating the epicardium and recruiting endogenous c‐kit(+) cells. Epicardial transplantation of the cell patch may be applied as a novel effective MI therapy. John Wiley and Sons Inc. 2017-02-28 2017-09 /pmc/articles/PMC5571540/ /pubmed/28244640 http://dx.doi.org/10.1111/jcmm.13097 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Wang, Qiang‐li Wang, Hai‐jie Li, Zhi‐hua Wang, Yong‐li Wu, Xue‐ping Tan, Yu‐zhen Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title | Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title_full | Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title_fullStr | Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title_full_unstemmed | Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title_short | Mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
title_sort | mesenchymal stem cell‐loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571540/ https://www.ncbi.nlm.nih.gov/pubmed/28244640 http://dx.doi.org/10.1111/jcmm.13097 |
work_keys_str_mv | AT wangqiangli mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium AT wanghaijie mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium AT lizhihua mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium AT wangyongli mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium AT wuxueping mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium AT tanyuzhen mesenchymalstemcellloadedcardiacpatchpromotesepicardialactivationandrepairoftheinfarctedmyocardium |