Cargando…
Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571746/ https://www.ncbi.nlm.nih.gov/pubmed/28660861 http://dx.doi.org/10.1107/S2053273317007902 |
_version_ | 1783259404266635264 |
---|---|
author | Krenkel, Martin Toepperwien, Mareike Alves, Frauke Salditt, Tim |
author_facet | Krenkel, Martin Toepperwien, Mareike Alves, Frauke Salditt, Tim |
author_sort | Krenkel, Martin |
collection | PubMed |
description | X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data. |
format | Online Article Text |
id | pubmed-5571746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-55717462017-09-05 Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime Krenkel, Martin Toepperwien, Mareike Alves, Frauke Salditt, Tim Acta Crystallogr A Found Adv Research Papers X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data. International Union of Crystallography 2017-06-29 /pmc/articles/PMC5571746/ /pubmed/28660861 http://dx.doi.org/10.1107/S2053273317007902 Text en © Martin Krenkel et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Research Papers Krenkel, Martin Toepperwien, Mareike Alves, Frauke Salditt, Tim Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title | Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title_full | Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title_fullStr | Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title_full_unstemmed | Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title_short | Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime |
title_sort | three-dimensional single-cell imaging with x-ray waveguides in the holographic regime |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571746/ https://www.ncbi.nlm.nih.gov/pubmed/28660861 http://dx.doi.org/10.1107/S2053273317007902 |
work_keys_str_mv | AT krenkelmartin threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime AT toepperwienmareike threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime AT alvesfrauke threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime AT salditttim threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime |