Cargando…

Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources...

Descripción completa

Detalles Bibliográficos
Autores principales: Krenkel, Martin, Toepperwien, Mareike, Alves, Frauke, Salditt, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571746/
https://www.ncbi.nlm.nih.gov/pubmed/28660861
http://dx.doi.org/10.1107/S2053273317007902
_version_ 1783259404266635264
author Krenkel, Martin
Toepperwien, Mareike
Alves, Frauke
Salditt, Tim
author_facet Krenkel, Martin
Toepperwien, Mareike
Alves, Frauke
Salditt, Tim
author_sort Krenkel, Martin
collection PubMed
description X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.
format Online
Article
Text
id pubmed-5571746
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-55717462017-09-05 Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime Krenkel, Martin Toepperwien, Mareike Alves, Frauke Salditt, Tim Acta Crystallogr A Found Adv Research Papers X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data. International Union of Crystallography 2017-06-29 /pmc/articles/PMC5571746/ /pubmed/28660861 http://dx.doi.org/10.1107/S2053273317007902 Text en © Martin Krenkel et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Krenkel, Martin
Toepperwien, Mareike
Alves, Frauke
Salditt, Tim
Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title_full Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title_fullStr Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title_full_unstemmed Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title_short Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
title_sort three-dimensional single-cell imaging with x-ray waveguides in the holographic regime
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571746/
https://www.ncbi.nlm.nih.gov/pubmed/28660861
http://dx.doi.org/10.1107/S2053273317007902
work_keys_str_mv AT krenkelmartin threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime
AT toepperwienmareike threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime
AT alvesfrauke threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime
AT salditttim threedimensionalsinglecellimagingwithxraywaveguidesintheholographicregime