ESCORTing proteins directly from whole cell-lysate for single-molecule studies

We have developed a method for Enzymatic Sortase-assisted Covalent Orientation-specific Restraint Tethering (ESCORT) recombinant proteins onto surfaces directly from cell-lysate. With an improved surface passivation method, we obviate the cumbersome purification steps even for single molecule studie...

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Detalles Bibliográficos
Autores principales: Srinivasan, Shwetha, Hazra, Jagadish P., Singaraju, Gayathri S., Deb, Debadutta, Rakshit, Sabyasachi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571901/
https://www.ncbi.nlm.nih.gov/pubmed/28756135
http://dx.doi.org/10.1016/j.ab.2017.07.022

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