DNA barcoding-based sexual association of Sovia lucasii and S. lii (Lepidoptera: Hesperiidae), with description of a new subspecies

Both sexes of two sympatric and sexually dimorphic species, Sovia lucasii (Mabille, 1876) and S. lii Xue, 2015, are associated based on DNA barcoding using the COI (mitochondrial cytochrome coxidase I) gene. The females are thus identified for the first time, and their wing patterns and genitalia are described and illustrated for the convenience of morphological identification hereafter. A new subspecies, S. lucasii minor ssp. nov., from the northeastern and eastern parts of the Sichuan Basin of China, is reported. External and genital differences between the new taxon and the nominate subspecies, which is distributed in western Sichuan and newly discovered in northwestern Guangxi, are illustrated and discussed.