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Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue

Diabetic foot ulcers (DFUs) often result in severely adverse outcomes, such as serious infections, hospitalization, and lower extremity amputations. In last few years, to improve the outcome of DFUs, clinicians and researchers put their attention on the application of low intensity pulsating electro...

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Autores principales: Ferroni, Letizia, Gardin, Chiara, Pieri, Andrea De, Sambataro, Maria, Seganfreddo, Elena, Iacopi, Elisabetta, Goretti, Chiara, Zavan, Barbara, Piaggesi, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572111/
https://www.ncbi.nlm.nih.gov/pubmed/29046049
http://dx.doi.org/10.4081/ejh.2017.2800
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author Ferroni, Letizia
Gardin, Chiara
Pieri, Andrea De
Sambataro, Maria
Seganfreddo, Elena
Iacopi, Elisabetta
Goretti, Chiara
Zavan, Barbara
Piaggesi, Alberto
author_facet Ferroni, Letizia
Gardin, Chiara
Pieri, Andrea De
Sambataro, Maria
Seganfreddo, Elena
Iacopi, Elisabetta
Goretti, Chiara
Zavan, Barbara
Piaggesi, Alberto
author_sort Ferroni, Letizia
collection PubMed
description Diabetic foot ulcers (DFUs) often result in severely adverse outcomes, such as serious infections, hospitalization, and lower extremity amputations. In last few years, to improve the outcome of DFUs, clinicians and researchers put their attention on the application of low intensity pulsating electro- magnetic fields through Therapeutic Magnetic Resonance (TMR(R)). In our study, patients with DFUs have been divided into two groups: The Sham Group treated with non-functioning TMR(R) device, and the Active Group treated with a functioning device. Biopsies were recovered from ulcers before and after a 15-day treatment with both kind of TMR(R) device. To recognize signs of inflammation or healing process, the harvested biopsies were subjected to histological and molecular analyses. The histological analysis showed a change in cell population after treatment with TMR(R): an increase of fibroblasts and endothelial cells with a reduction of inflammatory cells. After TMR(R) application, the gene expression profile analysis revealed an improvement in extracellular matrix components such as matrix metalloproteinases, collagens and integrins, a reduction in proinflammatory interleukins, and an increase in growth factors expression. In conclusion, our research has identified histological and molecular features of reduced inflammation and increased cell proliferation during the wound healing process in response to TMR(R) application.
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spelling pubmed-55721112017-09-01 Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue Ferroni, Letizia Gardin, Chiara Pieri, Andrea De Sambataro, Maria Seganfreddo, Elena Iacopi, Elisabetta Goretti, Chiara Zavan, Barbara Piaggesi, Alberto Eur J Histochem Original Paper Diabetic foot ulcers (DFUs) often result in severely adverse outcomes, such as serious infections, hospitalization, and lower extremity amputations. In last few years, to improve the outcome of DFUs, clinicians and researchers put their attention on the application of low intensity pulsating electro- magnetic fields through Therapeutic Magnetic Resonance (TMR(R)). In our study, patients with DFUs have been divided into two groups: The Sham Group treated with non-functioning TMR(R) device, and the Active Group treated with a functioning device. Biopsies were recovered from ulcers before and after a 15-day treatment with both kind of TMR(R) device. To recognize signs of inflammation or healing process, the harvested biopsies were subjected to histological and molecular analyses. The histological analysis showed a change in cell population after treatment with TMR(R): an increase of fibroblasts and endothelial cells with a reduction of inflammatory cells. After TMR(R) application, the gene expression profile analysis revealed an improvement in extracellular matrix components such as matrix metalloproteinases, collagens and integrins, a reduction in proinflammatory interleukins, and an increase in growth factors expression. In conclusion, our research has identified histological and molecular features of reduced inflammation and increased cell proliferation during the wound healing process in response to TMR(R) application. PAGEPress Publications, Pavia, Italy 2017-08-07 /pmc/articles/PMC5572111/ /pubmed/29046049 http://dx.doi.org/10.4081/ejh.2017.2800 Text en ©Copyright S. Salucci et al., 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Ferroni, Letizia
Gardin, Chiara
Pieri, Andrea De
Sambataro, Maria
Seganfreddo, Elena
Iacopi, Elisabetta
Goretti, Chiara
Zavan, Barbara
Piaggesi, Alberto
Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title_full Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title_fullStr Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title_full_unstemmed Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title_short Treatment of diabetic foot ulcers with Therapeutic Magnetic Resonance (TMR®) improves the quality of granulation tissue
title_sort treatment of diabetic foot ulcers with therapeutic magnetic resonance (tmr®) improves the quality of granulation tissue
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572111/
https://www.ncbi.nlm.nih.gov/pubmed/29046049
http://dx.doi.org/10.4081/ejh.2017.2800
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