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CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing

The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc f...

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Autores principales: Sattar, Muhammad N., Iqbal, Zafar, Tahir, Muhammad N., Shahid, Muhammad S., Khurshid, Muhammad, Al-Khateeb, Abdullatif A., Al-Khateeb, Suliman A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572371/
https://www.ncbi.nlm.nih.gov/pubmed/28878801
http://dx.doi.org/10.3389/fpls.2017.01469
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author Sattar, Muhammad N.
Iqbal, Zafar
Tahir, Muhammad N.
Shahid, Muhammad S.
Khurshid, Muhammad
Al-Khateeb, Abdullatif A.
Al-Khateeb, Suliman A.
author_facet Sattar, Muhammad N.
Iqbal, Zafar
Tahir, Muhammad N.
Shahid, Muhammad S.
Khurshid, Muhammad
Al-Khateeb, Abdullatif A.
Al-Khateeb, Suliman A.
author_sort Sattar, Muhammad N.
collection PubMed
description The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and most recently clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9)-based approaches have been successfully employed for various crop plants including fruit trees. CRISPR/Cas9-based approaches hold great potential in GE due to their simplicity, competency, and versatility over other GE techniques. However, to the best of our knowledge no such genetic improvement has ever been developed in date palm—an important fruit crop in Oasis agriculture. The applications of CRISPR/Cas9 can be a challenging task in date palm GE due to its large and complex genome, high rate of heterozygosity and outcrossing, in vitro regeneration and screening of mutants, high frequency of single-nucleotide polymorphism in the genome and ultimately genetic instability. In this review, we addressed the potential application of CRISPR/Cas9-based approaches in date palm GE to improve the sustainable date palm production. The availability of the date palm whole genome sequence has made it feasible to use CRISPR/Cas9 GE approach for genetic improvement in this species. Moreover, the future prospects of GE application in date palm are also addressed in this review.
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spelling pubmed-55723712017-09-06 CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing Sattar, Muhammad N. Iqbal, Zafar Tahir, Muhammad N. Shahid, Muhammad S. Khurshid, Muhammad Al-Khateeb, Abdullatif A. Al-Khateeb, Suliman A. Front Plant Sci Plant Science The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and most recently clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9)-based approaches have been successfully employed for various crop plants including fruit trees. CRISPR/Cas9-based approaches hold great potential in GE due to their simplicity, competency, and versatility over other GE techniques. However, to the best of our knowledge no such genetic improvement has ever been developed in date palm—an important fruit crop in Oasis agriculture. The applications of CRISPR/Cas9 can be a challenging task in date palm GE due to its large and complex genome, high rate of heterozygosity and outcrossing, in vitro regeneration and screening of mutants, high frequency of single-nucleotide polymorphism in the genome and ultimately genetic instability. In this review, we addressed the potential application of CRISPR/Cas9-based approaches in date palm GE to improve the sustainable date palm production. The availability of the date palm whole genome sequence has made it feasible to use CRISPR/Cas9 GE approach for genetic improvement in this species. Moreover, the future prospects of GE application in date palm are also addressed in this review. Frontiers Media S.A. 2017-08-23 /pmc/articles/PMC5572371/ /pubmed/28878801 http://dx.doi.org/10.3389/fpls.2017.01469 Text en Copyright © 2017 Sattar, Iqbal, Tahir, Shahid, Khurshid, Al-Khateeb and Al-Khateeb. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sattar, Muhammad N.
Iqbal, Zafar
Tahir, Muhammad N.
Shahid, Muhammad S.
Khurshid, Muhammad
Al-Khateeb, Abdullatif A.
Al-Khateeb, Suliman A.
CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title_full CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title_fullStr CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title_full_unstemmed CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title_short CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing
title_sort crispr/cas9: a practical approach in date palm genome editing
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572371/
https://www.ncbi.nlm.nih.gov/pubmed/28878801
http://dx.doi.org/10.3389/fpls.2017.01469
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