Cargando…
The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens
Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose (Morus alba) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isola...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572373/ https://www.ncbi.nlm.nih.gov/pubmed/28878804 http://dx.doi.org/10.3389/fpls.2017.01475 |
_version_ | 1783259514769768448 |
---|---|
author | Gai, Ying-Ping Zhao, Ya-Nan Zhao, Huai-Ning Yuan, Chuan-Zhong Yuan, Shuo-Shuo Li, Shuo Zhu, Bing-Sen Ji, Xian-Ling |
author_facet | Gai, Ying-Ping Zhao, Ya-Nan Zhao, Huai-Ning Yuan, Chuan-Zhong Yuan, Shuo-Shuo Li, Shuo Zhu, Bing-Sen Ji, Xian-Ling |
author_sort | Gai, Ying-Ping |
collection | PubMed |
description | Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose (Morus alba) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isolated from Husang 32 (M. multicaulis) and designated HMLX56. Amino acid sequence analysis and protein modeling of three MLX56 proteins showed that they were highly conserved among Morus species. Tissue expression pattern analysis showed that the HMLX56 gene was strongly expressed in mulberry bark and leaves but only slightly expressed in fruits. In addition, analysis of GUS expression indicated that the promoter of HMLX56 showed higher transcriptional activity along the vascular strands, and its activity can be regulated by various environmental factors. Like the MLX56 protein from M. alba, the HMLX56 protein showed toxicity to Plutella xylostella. Moreover, when the HMLX56 gene was ectopically expressed in Arabidopsis, the transgenic plants showed enhanced resistance to aphids, the fungal pathogen Botrytis cinerea and the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our data suggest that the HMLX56 protein has a lectin-like molecular structure consisting of two hevein-like chitin-binding domains which provide not only chitin-binding activities but also other mechanisms of defense. The information provided here improves our understanding of the potential functions and defense mechanisms of MLX56 proteins, enabling in-depth functional analysis of latex exudates and perhaps facilitating mulberry genetic improvement in the future. |
format | Online Article Text |
id | pubmed-5572373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55723732017-09-06 The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens Gai, Ying-Ping Zhao, Ya-Nan Zhao, Huai-Ning Yuan, Chuan-Zhong Yuan, Shuo-Shuo Li, Shuo Zhu, Bing-Sen Ji, Xian-Ling Front Plant Sci Plant Science Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose (Morus alba) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isolated from Husang 32 (M. multicaulis) and designated HMLX56. Amino acid sequence analysis and protein modeling of three MLX56 proteins showed that they were highly conserved among Morus species. Tissue expression pattern analysis showed that the HMLX56 gene was strongly expressed in mulberry bark and leaves but only slightly expressed in fruits. In addition, analysis of GUS expression indicated that the promoter of HMLX56 showed higher transcriptional activity along the vascular strands, and its activity can be regulated by various environmental factors. Like the MLX56 protein from M. alba, the HMLX56 protein showed toxicity to Plutella xylostella. Moreover, when the HMLX56 gene was ectopically expressed in Arabidopsis, the transgenic plants showed enhanced resistance to aphids, the fungal pathogen Botrytis cinerea and the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our data suggest that the HMLX56 protein has a lectin-like molecular structure consisting of two hevein-like chitin-binding domains which provide not only chitin-binding activities but also other mechanisms of defense. The information provided here improves our understanding of the potential functions and defense mechanisms of MLX56 proteins, enabling in-depth functional analysis of latex exudates and perhaps facilitating mulberry genetic improvement in the future. Frontiers Media S.A. 2017-08-23 /pmc/articles/PMC5572373/ /pubmed/28878804 http://dx.doi.org/10.3389/fpls.2017.01475 Text en Copyright © 2017 Gai, Zhao, Zhao, Yuan, Yuan, Li, Zhu and Ji. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Gai, Ying-Ping Zhao, Ya-Nan Zhao, Huai-Ning Yuan, Chuan-Zhong Yuan, Shuo-Shuo Li, Shuo Zhu, Bing-Sen Ji, Xian-Ling The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title | The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title_full | The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title_fullStr | The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title_full_unstemmed | The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title_short | The Latex Protein MLX56 from Mulberry (Morus multicaulis) Protects Plants against Insect Pests and Pathogens |
title_sort | latex protein mlx56 from mulberry (morus multicaulis) protects plants against insect pests and pathogens |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572373/ https://www.ncbi.nlm.nih.gov/pubmed/28878804 http://dx.doi.org/10.3389/fpls.2017.01475 |
work_keys_str_mv | AT gaiyingping thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhaoyanan thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhaohuaining thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT yuanchuanzhong thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT yuanshuoshuo thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT lishuo thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhubingsen thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT jixianling thelatexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT gaiyingping latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhaoyanan latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhaohuaining latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT yuanchuanzhong latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT yuanshuoshuo latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT lishuo latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT zhubingsen latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens AT jixianling latexproteinmlx56frommulberrymorusmulticaulisprotectsplantsagainstinsectpestsandpathogens |